Stem Cell Res Ther. 2013;4 Suppl 1(Suppl 1):S3. doi: 10.1186/scrt364. Epub 2013 Dec 20.
Researchers need an available and easy-to-use model of the human intestine to better understand human intestinal physiology and pathophysiology of diseases, and to offer an enhanced platform for developing drug therapy. Our work employs human enteroids derived from each of the major intestinal sections to advance understanding of several diarrheal diseases, including those caused by cholera, rotavirus and enterohemorrhagic Escherichia coli. An enteroid bank is being established to facilitate comparison of segmental, developmental, and regulatory differences in transport proteins that can influence therapy efficacy. Basic characterization of major ion transport protein expression, localization and function in the human enteroid model sets the stage to study the effects of enteric infection at the transport level, as well as to monitor potential responses to pharmacological intervention.
研究人员需要一个可用且易于使用的人类肠道模型，以便更好地了解人类肠道生理学和疾病的病理生理学，并为开发药物治疗提供一个增强的平台。我们的工作利用源自每个主要肠道段的人类类肠组织，来深入了解几种腹泻病，包括霍乱、轮状病毒和肠出血性大肠杆菌引起的腹泻病。正在建立一个类肠组织库，以方便比较影响治疗效果的转运蛋白在节段性、发育性和调节性方面的差异。人类类肠组织模型中主要离子转运蛋白表达、定位和功能的基本特征为在转运水平上研究肠内感染的影响以及监测对潜在药物干预的反应奠定了基础。
