Bulit Florence, Grad Iwona, Manoil Daniel, Simon Stéphane, Wataha John C, Filieri Anna, Feki Anis, Schrenzel Jacques, Lange Norbert, Bouillaguet Serge
Department of Cariology and Endodontology, School of Dental Medicine, University of Geneva, Geneva, Switzerland.
Department of Endodontics, School of Dentistry, University of Paris Diderot, Paris, France.
J Endod. 2014 Mar;40(3):427-31. doi: 10.1016/j.joen.2013.12.001. Epub 2014 Jan 10.
Pulp repair is less likely to occur when dentin or pulpal tissue remains infected after caries excavation. Yet there are currently few options to kill residual bacteria without damaging resident cells. The current study has evaluated the effect of 3 blue light-activated chemicals on the viability of lactobacilli, odontoblast-like cells (MDPC-23), undifferentiated pulp cells (OD21), and human embryonic stem cells (hESC H1).
Bacteria were incubated for 15 minutes with curcumin, eosin Y, or rose bengal and then irradiated with blue light (240 seconds). Bacteria were labeled with LIVE/DEAD BacLight Bacterial Viability kit, and viability was assessed by fluorescence-activated cell sorting. Cytotoxicity assays were performed on MDPC-23 cells, OD21, and hESC H1 cells grown in 24-well plates and exposed to the same photosensitizer-light combination. After 24 hours, cellular response was measured by using the methyl-thiazol-diphenyl-tetrazolium assay. Results were statistically analyzed by using one-way analysis of variance and Tukey multiple comparison intervals (α = 0.05).
Bacterial viability was significantly reduced after exposure to different combinations of light and photosensitizers; mitochondrial activity of cultured cells remained unaffected when exposed to the same conditions, suggesting a good therapeutic index in vitro.
Blue light-mediated disinfection is promising for the development of new treatment strategies designed to promote pulp repair after carious exposure.
龋洞挖除后牙本质或牙髓组织仍存在感染时，牙髓修复的可能性较小。然而，目前在不损伤驻留细胞的情况下杀灭残留细菌的方法很少。本研究评估了3种蓝光激活化学物质对乳酸杆菌、成牙本质细胞样细胞（MDPC-23）、未分化牙髓细胞（OD21）和人胚胎干细胞（hESC H1）活力的影响。
将细菌与姜黄素、伊红Y或孟加拉玫瑰红孵育15分钟，然后用蓝光照射（240秒）。用LIVE/DEAD BacLight细菌活力试剂盒标记细菌，并通过荧光激活细胞分选评估活力。对在24孔板中培养并暴露于相同光敏剂-光组合的MDPC-23细胞、OD21细胞和hESC Hl细胞进行细胞毒性试验。24小时后，使用甲基噻唑二苯基四氮唑试验测量细胞反应。结果采用单因素方差分析和Tukey多重比较区间进行统计学分析（α=0.05）。
暴露于不同的光和光敏剂组合后，细菌活力显著降低；在相同条件下，培养细胞的线粒体活性未受影响，表明在体外具有良好的治疗指数。
蓝光介导的消毒有望用于开发旨在促进龋病暴露后牙髓修复的新治疗策略。
