Mandroli Praveenkumar S, Prabhakar A R, Bhat Kishore, Krishnamurthy Sushma, Bogar Chetana
Department of Pedodontics and Preventive Dentistry, Belgaum, Karnataka, India.
Department of Pedodontics and Preventive Dentistry, Bapuji Dental College and Hospital, Davangere, Karnataka, India.
Ayu. 2019 Jul-Sep;40(3):192-195. doi: 10.4103/ayu.AYU_294_18. Epub 2020 Aug 8.
Curcumin, a component of turmeric ( L.), is a molecule of multitude of medicinal properties. Although curcumin has found a place in the treatment of gingival and periodontal diseases, there are no reported cytotoxicity studies on the cells of clinical significance (i.e., periodontal ligament [PDL] fibroblasts).
The objective of this research was to assess the cytotoxicity of curcumin against human PDL fibroblasts.
Human PDL fibroblasts from premolar teeth were cultured and used for cytotoxicity tests from healthy children presented for orthodontic extractions. Test concentrations of curcumin (100%, 50%, and 25%) were prepared by diluting 95% curcumin with di‑methyl‑sulfoxide and added to 96‑well microtiter plate (in triplicate) containing the fibroblast culture (approximately 2 × 104 cells/well). Fibroblast cells without treatment (without curcumin) acted as a control group. The viability of cells after 48 h of incubation at 37°C in a humidified atmosphere of 5% CO and 95% air was ascertained by the 3‑(4, 5‑dimethyl‑thiazol‑2‑yl)‑2, 5‑diphenyl‑tetrazolium bromide (MTT) assay. The viability of PDL fibroblast cells of experimental wells was expressed relative to that of control, in terms of change in the color intensity. Absorbencies were recorded at 450 nm on a microplate reader with background subtraction at 620 nm. The cell viability at various concentrations of curcumin against the PDL fibroblasts was calculated as mean absorbance (optical density) and percentage values.
Cell viability of PDL fibroblasts to 100%, 50%, and 25% curcumin concentration was 111.75%, 112.50%, and 114.40%, respectively.
No cytotoxicity was detected for curcumin against human PDL fibroblasts, at any of the concentrations used (100%, 50%, and 25%) by MTT assay at the end of 48 h.
姜黄素是姜黄（Curcuma longa L.）的一种成分，是具有多种药用特性的分子。尽管姜黄素已在牙龈和牙周疾病的治疗中占有一席之地，但尚无关于具有临床意义的细胞（即牙周膜[PDL]成纤维细胞）的细胞毒性研究报道。
本研究的目的是评估姜黄素对人牙周膜成纤维细胞的细胞毒性。
从健康儿童因正畸拔牙而拔除的前磨牙中获取人牙周膜成纤维细胞，进行培养并用于细胞毒性试验。通过用二甲基亚砜稀释95%的姜黄素来制备测试浓度的姜黄素（100%、50%和25%），并将其添加到含有成纤维细胞培养物（约2×104个细胞/孔）的96孔微量滴定板中（一式三份）。未处理（无姜黄素）的成纤维细胞作为对照组。在37°C、5%二氧化碳和95%空气的湿润气氛中孵育48小时后，通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑（MTT）法确定细胞活力。根据颜色强度的变化，将实验孔中PDL成纤维细胞的活力相对于对照组进行表示。在酶标仪上于450nm处记录吸光度，并在620nm处扣除背景。计算不同浓度姜黄素对PDL成纤维细胞的细胞活力，以平均吸光度（光密度）和百分比值表示。
PDL成纤维细胞对100%、50%和25%姜黄素浓度的细胞活力分别为111.75%、112.50%和114.40%。
在48小时结束时，通过MTT法在任何使用的浓度（100%、50%和25%）下均未检测到姜黄素对人PDL成纤维细胞的细胞毒性。
