Taga H, Hirai H, Ishizuka H, Kaneda H
Tumour Laboratory, Nihon University Surugadai Hospital, Tokyo, Japan.
Tumour Biol. 1988;9(2-3):110-5. doi: 10.1159/000217551.
A sensitive procedure involving lectin affinity electrophoresis of alpha-fetoprotein (AFP) was established. AFPs electrophoresed on lectin-containing gels were blotted on nitrocellulose membrane which was precoated with the specific antibody to AFP and stained with peroxidase-labeled anti-AFP antibody. This method could detect as little as 4 micrograms/l of purified AFP dissolved in buffer, or 50 micrograms/l in serum specimens. A number of patients with liver disease have been followed for long periods in Nihon University Hospital, Tokyo. Serum specimens were collected serially and stored frozen. We have reinvestigated retrospectively 6 series of serum specimens by the lectin-immunoblotting technique and found 3 cases that revealed a hepatocellular carcinoma-specific AFP variant at a very early stage, in advance of any other evidence of hepatocellular carcinoma by clinical examination.
建立了一种涉及甲胎蛋白（AFP）凝集素亲和电泳的灵敏方法。在含凝集素的凝胶上进行电泳的AFP被印迹到预先用AFP特异性抗体包被的硝酸纤维素膜上，并用过氧化物酶标记的抗AFP抗体染色。该方法能够检测到溶解在缓冲液中的低至4微克/升的纯化AFP，或血清标本中50微克/升的AFP。东京日本大学医院对许多肝病患者进行了长期随访。连续采集血清标本并冷冻保存。我们通过凝集素免疫印迹技术对6组血清标本进行了回顾性重新研究，发现3例在肝细胞癌的任何其他临床检查证据出现之前的非常早期就显示出肝细胞癌特异性AFP变体的病例。
