Danscher G
Stain Technol. 1983 Nov;58(6):365-72. doi: 10.3109/10520298309066814.
This report presents a method which can be used for counterstaining semithin sections of plastic embedded tissue. The sections are treated with a solution of silver lactate, followed by physical development. During the silver lactate treatment, silver ions are bound by various tissue components as metallic silver or silver sulfide. During physical development catalytic reduction of silver ions to metallic silver takes place where silver has been bound in the tissue, enlarging the silver deposits to microscopically visible dimensions. The amplified silver deposits give high contrast staining in yellow, brown and black suitable for both color and monochrome photography. The localization of the silver deposits is highly specific and may reflect several independent chemical processes. Examples in several tissues are shown.
本报告介绍了一种可用于对塑料包埋组织的半薄切片进行复染的方法。切片先用乳酸银溶液处理，然后进行物理显影。在乳酸银处理过程中，银离子以金属银或硫化银的形式与各种组织成分结合。在物理显影过程中，在组织中已结合银的部位发生银离子催化还原为金属银的反应，使银沉积物扩大到显微镜可见的尺寸。放大后的银沉积物呈现出适合彩色和黑白摄影的黄色、棕色和黑色高对比度染色。银沉积物的定位具有高度特异性，可能反映了几个独立的化学过程。文中展示了几种组织中的实例。
