Klinman D M, Banks S, Hartman A, Steinberg A D
Cellular Immunology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, Maryland 20892.
J Clin Invest. 1988 Aug;82(2):652-7. doi: 10.1172/JCI113644.
Splenic B cells from normal and autoimmune mice were transferred to MHC-compatible xid recipients. Monoclonal antibodies were secreted by the transferred B cells in splenic fragment cultures. These antibodies were evaluated for reactivity and cross-reactivity against a panel of six autoantigens and two conventional antigens using an ELISA assay. The autoantibodies and conventional antibodies produced in splenic fragment cultures by normal DBA/2 and autoimmune NZB B cells expressed similar degrees of antigenic cross-reactivity. Previous studies have demonstrated that ELISA assays of splenic fragment culture supernatants detect antibodies with affinities of 5 x 10(6) M-1 or greater. We therefore also analyzed the cross-reactivity of monoclonal antibodies derived from hybridomas. This permitted an assessment of antibodies with lower binding affinities. Cross-reactivity was detected more frequently among these hybridomas. Consistent with our earlier observations, hybridoma antibodies specific for conventional antigens exhibited cross-reactivity with a frequency similar to that of antibodies specific for autoantigens.
将正常小鼠和自身免疫小鼠的脾脏B细胞转移至主要组织相容性复合体（MHC）兼容的xid受体小鼠体内。在脾脏片段培养物中，转移的B细胞分泌出单克隆抗体。使用酶联免疫吸附测定（ELISA）法，评估这些抗体对一组六种自身抗原和两种传统抗原的反应性和交叉反应性。正常DBA/2和自身免疫性NZB B细胞在脾脏片段培养物中产生的自身抗体和传统抗体表现出相似程度的抗原交叉反应性。先前的研究表明，对脾脏片段培养上清液进行ELISA检测可检测到亲和力为5×10⁶ M⁻¹或更高的抗体。因此，我们还分析了源自杂交瘤的单克隆抗体的交叉反应性。这使得能够评估具有较低结合亲和力的抗体。在这些杂交瘤中更频繁地检测到交叉反应性。与我们早期的观察结果一致，对传统抗原具有特异性的杂交瘤抗体表现出交叉反应性的频率与对自身抗原具有特异性的抗体相似。
