Klinman D M, Ishigatsubo Y, Steinberg A D
Cellular Immunology Section, National Institute of Arthritis, Bethesda, MD 20892.
J Immunol. 1988 Aug 1;141(3):801-6.
The expressed B cell repertoires of mice from 1 day to 5 mo of age were examined. The ELISA-spot assay was used to enumerate individual Ig-secreting splenic B cells and determine the proportion of these cells producing antibodies reactive with each of six autoantigens and two conventional Ag. Results indicate that i) neonatal B cells producing antibodies against specific members of the Ag panel arose in a temporally defined sequence, ii) antibodies produced by 1- to 5-wk-old mice appeared to be more cross-reactive than those produced by adult mice, iii) no bias toward autoantibody production was found in the neonatal repertoires of autoimmune-prone mice, and iv) as a whole, the pattern of repertoire development among diverse strains of mice was highly conserved, although individual mice varied considerably in the absolute number of B cells committed to the production of antibodies of a given specificity.
对1日龄至5月龄小鼠的表达B细胞库进行了检测。采用酶联免疫斑点分析来计数分泌免疫球蛋白的脾脏B细胞个体，并确定这些细胞产生与六种自身抗原和两种传统抗原中的每一种发生反应的抗体的比例。结果表明：i）产生针对抗原组特定成员抗体的新生B细胞按时间定义的顺序出现；ii）1至5周龄小鼠产生的抗体似乎比成年小鼠产生的抗体具有更强的交叉反应性；iii）在易患自身免疫病小鼠的新生B细胞库中未发现偏向自身抗体产生的情况；iv）总体而言，尽管不同品系小鼠中致力于产生特定特异性抗体的B细胞绝对数量差异很大，但小鼠不同品系间的B细胞库发育模式高度保守。
