Klinman D M, Holmes K L, Conover J, Chiang B L, Gershwin M E
Section of Retroviral Immunology, CBER, FDA Bethesda, MD 20892.
Eur J Immunol. 1993 Aug;23(8):1866-71. doi: 10.1002/eji.1830230820.
NZB.H-2bm12 mice develop an autoimmune syndrome characterized by the overproduction of anti-DNA antibodies and the expansion of B-1 B cells. Thus, these animals provide a useful model to examine the antigenic specificity, cross-reactivity and functional capability of B-1 versus conventional lymphocytes. Neither the repertoire expressed by in vivo activated Ly-1+ splenic lymphocytes, nor their cross-reactivity, differed significantly from that of conventional splenic B cells. When Ly-1+ cells were cultured in vitro in the presence of lipopolysaccharide plus interleukin-4 or interferon gamma, they underwent isotype switching at the same frequency as conventional B cells. Of interest, B-1 cells from the peritoneal cavity were significantly less likely to undergo isotype switching than those from the spleen. These findings indicate that in vivo activated B-1a and conventional B cells from mice with lupus manifest similar functional characteristics.
NZB.H-2bm12小鼠会患上一种自身免疫综合征，其特征为抗DNA抗体过度产生以及B-1 B细胞扩增。因此，这些动物为研究B-1淋巴细胞与传统淋巴细胞的抗原特异性、交叉反应性和功能能力提供了一个有用的模型。体内活化的Ly-1+脾淋巴细胞所表达的库及其交叉反应性，与传统脾B细胞相比均无显著差异。当Ly-1+细胞在脂多糖加白细胞介素-4或干扰素γ存在的情况下进行体外培养时，它们发生同种型转换的频率与传统B细胞相同。有趣的是，来自腹腔的B-1细胞发生同种型转换的可能性明显低于来自脾脏的B-1细胞。这些发现表明，来自狼疮小鼠的体内活化B-1a细胞和传统B细胞表现出相似的功能特征。
