Nawa Yasunori, Inami Wataru, Miyake Aki, Ono Atsushi, Kawata Yoshimasa, Lin Sheng, Terakawa Susumu
Graduate School of Science and Technology, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; Research Fellow of the Japan Society for the Promotion of Science, Chiyoda, Tokyo 102-0083, Japan.
Faculty of Engineering, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; CREST, Japan Science and Technology Agency, Japan.
Biomed Opt Express. 2014 Jan 7;5(2):378-86. doi: 10.1364/BOE.5.000378. eCollection 2014 Feb 1.
We developed a high-resolution fluorescence microscope in which fluorescent materials are directly excited using a focused electron beam. Electron beam excitation enables detailed observations on the nanometer scale. Real-time live-cell observation is also possible using a thin film to separate the environment under study from the vacuum region required for electron beam propagation. In this study, we demonstrated observation of cellular components by autofluorescence excited with a focused electron beam and performed dynamic observations of intracellular granules. Since autofluorescence is associated with endogenous substances in cells, this microscope can also be used to investigate the intrinsic properties of organelles.
我们开发了一种高分辨率荧光显微镜，其中荧光材料通过聚焦电子束直接激发。电子束激发能够在纳米尺度上进行详细观察。利用薄膜将研究环境与电子束传播所需的真空区域隔开，还可以进行实时活细胞观察。在本研究中，我们展示了通过聚焦电子束激发的自发荧光对细胞成分进行观察，并对细胞内颗粒进行了动态观察。由于自发荧光与细胞内的内源性物质相关，这种显微镜还可用于研究细胞器的内在特性。
