1] Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, Belgium. [2] Center for Nano- and Biophotonics, Ghent University, Ghent, Belgium.
1] Department of Nanophysics, Italian Institute of Technology, Genoa, Italy. [2] Department of Physics, University of Genoa, Genoa, Italy.
Nat Methods. 2014 Mar;11(3):253-66. doi: 10.1038/nmeth.2843.
Methods based on single-molecule localization and photophysics have brought nanoscale imaging with visible light into reach. This has enabled single-particle tracking applications for studying the dynamics of molecules and nanoparticles and contributed to the recent revolution in super-resolution localization microscopy techniques. Crucial to the optimization of such methods are the precision and accuracy with which single fluorophores and nanoparticles can be localized. We present a lucid synthesis of the developments on this localization precision and accuracy and their practical implications in order to guide the increasing number of researchers using single-particle tracking and super-resolution localization microscopy.
基于单分子定位和光物理的方法已经将可见光下的纳米级成像变为可能。这使得单粒子追踪应用得以实现，可用于研究分子和纳米粒子的动力学，并推动了超分辨率定位显微镜技术的最新革命。这些方法的优化关键在于单荧光团和纳米粒子的定位精度和准确性。我们对这些定位精度和准确性的发展进行了清晰的综合，并对其在指导越来越多使用单粒子追踪和超分辨率定位显微镜的研究人员方面的实际意义进行了探讨。
