Departments of Chemistry and ‡Electrical Engineering, Stanford University , Stanford, California 94305, United States.
Nano Lett. 2013 Sep 11;13(9):3967-72. doi: 10.1021/nl304359p. Epub 2013 Feb 6.
The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle α > 60° can mislocalization errors be bounded to ≤10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.
单分子（SM）偶极子发射模式的不对称性限制了基于定位的超分辨率荧光显微镜中位置确定的准确性。定位误差的程度高度依赖于 SM 的旋转迁移率；只有当 SM 在旋转时的圆锥半角 α > 60°，才能将定位误差限制在 ≤10nm 以内。模拟演示了低或高旋转迁移率如何导致超分辨率重建中的分辨率降低或失真。
