单分子的旋转迁移率会影响超分辨率荧光显微镜中的定位精度。
Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy.
机构信息
Departments of Chemistry and ‡Electrical Engineering, Stanford University , Stanford, California 94305, United States.
出版信息
Nano Lett. 2013 Sep 11;13(9):3967-72. doi: 10.1021/nl304359p. Epub 2013 Feb 6.
Abstract
The asymmetric nature of single-molecule (SM) dipole emission patterns limits the accuracy of position determination in localization-based super-resolution fluorescence microscopy. The degree of mislocalization depends highly on the rotational mobility of SMs; only for SMs rotating within a cone half angle α > 60° can mislocalization errors be bounded to ≤10 nm. Simulations demonstrate how low or high rotational mobility can cause resolution degradation or distortion in super-resolution reconstructions.
摘要
单分子（SM）偶极子发射模式的不对称性限制了基于定位的超分辨率荧光显微镜中位置确定的准确性。定位误差的程度高度依赖于 SM 的旋转迁移率；只有当 SM 在旋转时的圆锥半角 α > 60°，才能将定位误差限制在 ≤10nm 以内。模拟演示了低或高旋转迁移率如何导致超分辨率重建中的分辨率降低或失真。
相似文献
1
Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy.单分子的旋转迁移率会影响超分辨率荧光显微镜中的定位精度。
Nano Lett. 2013 Sep 11;13(9):3967-72. doi: 10.1021/nl304359p. Epub 2013 Feb 6.
2
The role of molecular dipole orientation in single-molecule fluorescence microscopy and implications for super-resolution imaging.分子偶极取向在单分子荧光显微镜中的作用及其对超分辨率成像的影响。
Chemphyschem. 2014 Mar 17;15(4):587-99. doi: 10.1002/cphc.201300880. Epub 2013 Dec 30.
3
Deep learning massively accelerates super-resolution localization microscopy.深度学习极大地加速了超分辨率定位显微镜。
Nat Biotechnol. 2018 Jun;36(5):460-468. doi: 10.1038/nbt.4106. Epub 2018 Apr 16.
4
Ultrasensitive Three-Dimensional Orientation Imaging of Single Molecules on Plasmonic Nanohole Arrays Using Second Harmonic Generation.利用二次谐波产生技术在等离子体纳米孔阵列上对单个分子进行超灵敏三维取向成像。
Nano Lett. 2019 Sep 11;19(9):6192-6202. doi: 10.1021/acs.nanolett.9b02239. Epub 2019 Aug 12.
5
The double-helix point spread function enables precise and accurate measurement of 3D single-molecule localization and orientation.双螺旋点扩散函数能够对三维单分子定位和方向进行精确测量。
Proc SPIE Int Soc Opt Eng. 2013 Feb 22;8590:85900. doi: 10.1117/12.2001671.
6
Azimuthal polarization filtering for accurate, precise, and robust single-molecule localization microscopy.用于精确、准确和稳健的单分子定位显微镜的方位角偏振滤波
Nano Lett. 2014 Nov 12;14(11):6407-13. doi: 10.1021/nl502914k. Epub 2014 Oct 16.
7
Nanoscale separation of molecular species based on their rotational mobility.基于分子旋转迁移率的纳米级分子分离。
Opt Express. 2008 Dec 8;16(25):21093-104. doi: 10.1364/oe.16.021093.
8
Super-Resolving the Actual Position of Single Fluorescent Molecules Coupled to a Plasmonic Nanoantenna.超分辨单荧光分子与等离子体纳米天线耦合的实际位置。
ACS Nano. 2017 Sep 26;11(9):8978-8987. doi: 10.1021/acsnano.7b03420. Epub 2017 Aug 18.
9
Enhanced 4Pi single-molecule localization microscopy with coherent pupil based localization.基于相干光瞳定位的增强型 4Pi 单分子定位显微镜
Commun Biol. 2020 May 8;3(1):220. doi: 10.1038/s42003-020-0908-2.
10
Recent advances in super-resolution fluorescence imaging and its applications in biology.超分辨率荧光成像技术的最新进展及其在生物学中的应用。
J Genet Genomics. 2013 Dec 20;40(12):583-95. doi: 10.1016/j.jgg.2013.11.003. Epub 2013 Nov 23.
引用本文的文献
1
Resolving the Orientations of and Angular Separation Between a Pair of Dipole Emitters.解析一对偶极发射器的方向及角间距。
Phys Rev Lett. 2025 Mar 7;134(9):093805. doi: 10.1103/PhysRevLett.134.093805.
2
Single-molecule orientation-localization microscopy: Applications and approaches.单分子取向定位显微镜：应用与方法
Q Rev Biophys. 2024 Dec 23;57:e17. doi: 10.1017/S0033583524000167.
3
Improved localization precision via restricting confined biomolecule stochastic motion in single-molecule localization microscopy.通过在单分子定位显微镜中限制受限生物分子的随机运动提高定位精度。
Nanophotonics. 2021 Nov 16;11(1):53-65. doi: 10.1515/nanoph-2021-0481. eCollection 2022 Jan.
4
Combining deep learning approaches and point spread function engineering for simultaneous 3D position and 3D orientation measurements of fluorescent single molecules.结合深度学习方法和点扩散函数工程实现荧光单分子的三维位置和三维方向的同步测量。
Opt Commun. 2023 Sep 1;542. doi: 10.1016/j.optcom.2023.129589. Epub 2023 May 11.
5
Measurement of Molecular Height Using Cell Surface Optical Profilometry (CSOP).使用细胞表面光学轮廓术（CSOP）测量分子高度。
Methods Mol Biol. 2023;2654:113-122. doi: 10.1007/978-1-0716-3135-5_7.
6
Photon efficient orientation estimation using polarization modulation in single-molecule localization microscopy.单分子定位显微镜中基于偏振调制的光子高效取向估计
Biomed Opt Express. 2022 Apr 21;13(5):2835-2858. doi: 10.1364/BOE.452159. eCollection 2022 May 1.
7
Resolving the Three-Dimensional Rotational and Translational Dynamics of Single Molecules Using Radially and Azimuthally Polarized Fluorescence.利用径向和角向偏振荧光解析单分子的三维旋转和平移动力学。
Nano Lett. 2022 Feb 9;22(3):1024-1031. doi: 10.1021/acs.nanolett.1c03948. Epub 2022 Jan 24.
8
4polar-STORM polarized super-resolution imaging of actin filament organization in cells.4 极 -STORM 极化超分辨率成像技术在细胞中肌动蛋白丝组织的研究。
Nat Commun. 2022 Jan 13;13(1):301. doi: 10.1038/s41467-022-27966-w.
9
Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble Using a Polarized Vortex Point Spread Function.利用偏振涡旋点扩散函数进行 3D 取向和各向异性摆动的单分子定位显微镜。
J Phys Chem B. 2021 Nov 25;125(46):12718-12729. doi: 10.1021/acs.jpcb.1c08073. Epub 2021 Nov 12.
10
Simultaneous orientation and 3D localization microscopy with a Vortex point spread function.利用涡旋点扩散函数进行同时定位和 3D 定位显微镜。
Nat Commun. 2021 Oct 11;12(1):5934. doi: 10.1038/s41467-021-26228-5.
本文引用的文献
1
Simultaneous, accurate measurement of the 3D position and orientation of single molecules.同时精确测量单个分子的 3D 位置和方向。
Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19087-92. doi: 10.1073/pnas.1216687109. Epub 2012 Nov 5.
2
Ultrabright photoactivatable fluorophores created by reductive caging.通过还原笼蔽作用产生的超高亮光活化荧光团。
Nat Methods. 2012 Dec;9(12):1181-4. doi: 10.1038/nmeth.2214. Epub 2012 Oct 28.
3
Microscopy beyond the diffraction limit using actively controlled single molecules.利用主动控制的单分子实现超越衍射极限的显微镜技术。
J Microsc. 2012 Jun;246(3):213-20. doi: 10.1111/j.1365-2818.2012.03600.x. Epub 2012 Apr 12.
4
Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging.基于定位的超分辨率成像中荧光团最佳性能的评估。
Nat Methods. 2011 Nov 6;8(12):1027-36. doi: 10.1038/nmeth.1768.
5
Accuracy of the gaussian point spread function model in 2D localization microscopy.二维定位显微镜中高斯点扩散函数模型的准确性。
Opt Express. 2010 Nov 22;18(24):24461-76. doi: 10.1364/OE.18.024461.
6
Molecular orientation affects localization accuracy in superresolution far-field fluorescence microscopy.分子取向会影响远场荧光显微镜超分辨率定位的准确性。
Nano Lett. 2011 Jan 12;11(1):209-13. doi: 10.1021/nl103472b. Epub 2010 Dec 6.
7
Fluorescence lifetime measurements and biological imaging.荧光寿命测量与生物成像。
Chem Rev. 2010 May 12;110(5):2641-84. doi: 10.1021/cr900343z.
8
Quantitative imaging of molecular order in lipid membranes using two-photon fluorescence polarimetry.使用双光子荧光偏振法定量成像脂质膜中的分子有序性。
Biophys J. 2009 Nov 18;97(10):2854-62. doi: 10.1016/j.bpj.2009.08.052.
9
Polarization effect on position accuracy of fluorophore localization.极化对荧光团定位位置精度的影响。
Opt Express. 2006 Sep 4;14(18):8111-20. doi: 10.1364/oe.14.008111.
10
Microscopy and its focal switch.显微镜及其焦点切换。
Nat Methods. 2009 Jan;6(1):24-32. doi: 10.1038/nmeth.1291.
Zotero 插件