Hashim Puziah
Halal Products Research Institute, Universiti Putra Malaysia, Putra Infoport, UPM Serdang, Selangor, Malaysia.
Pak J Pharm Sci. 2014 Mar;27(2):233-7.
Centella asiatica (Linn.) Urban is well known in promoting wound healing and provides significant benefits in skin care and therapeutic products formulation. Glycolic acid and vitamins also play a role in the enhancement of collagen and fibronectin synthesis. Here, we evaluate the specific effect of Centella asiatica (CA), vitamins, glycolic acid and their mixture preparations to stimulate collagen and fibronectin synthesis in cultured human fibroblast cells. The fibroblast cells are incubated with CA, glycolic acid, vitamins and their mixture preparations for 48 h. The cell lysates were analyzed for protein content and collagen synthesis by direct binding enzyme immunoassay. The fibronectin of the cultured supernatant was measured by sandwich enzyme immunoassay. The results showed that CA, glycolic acid, vitamins A, E and C significantly stimulate collagen and fibronectin synthesis in the fibroblast. Addition of glycolic acid and vitamins to CA further increased the levels of collagen and fibronectin synthesis to 8.55 and 23.75 μg/100 μg, respectively. CA, glycolic acid, vitamins A, E, and C, and their mixtures demonstrated stimulatory effect on both extra-cellular matrix synthesis of collagen and fibronectin in in vitro studies on human foreskin fibroblasts, which is beneficial to skin care and therapeutic products formulation.
积雪草（Centella asiatica (Linn.) Urban）在促进伤口愈合方面广为人知，并且在护肤品和治疗产品配方中具有显著益处。乙醇酸和维生素在增强胶原蛋白和纤连蛋白合成方面也发挥作用。在此，我们评估积雪草（CA）、维生素、乙醇酸及其混合制剂对培养的人成纤维细胞中胶原蛋白和纤连蛋白合成的具体影响。将成纤维细胞与CA、乙醇酸、维生素及其混合制剂孵育48小时。通过直接结合酶免疫测定法分析细胞裂解物中的蛋白质含量和胶原蛋白合成。通过夹心酶免疫测定法测量培养上清液中的纤连蛋白。结果表明，CA、乙醇酸、维生素A、E和C显著刺激成纤维细胞中胶原蛋白和纤连蛋白的合成。向CA中添加乙醇酸和维生素进一步将胶原蛋白和纤连蛋白的合成水平分别提高到8.55和23.75μg/100μg。在对人包皮成纤维细胞的体外研究中，CA、乙醇酸、维生素A、E和C及其混合物对胶原蛋白和纤连蛋白的细胞外基质合成均表现出刺激作用，这有利于护肤品和治疗产品配方。
