Kim S J, Won Y H
Department of Dermatology, Chonnam University Research Institute of Medical Science, Chonnam National University Medical School, Kwangju, Korea.
J Dermatol. 1998 Feb;25(2):85-9.
Glycolic acid peeling is known to improve photoaging processes such as wrinkling and roughness, but this effect has not been clearly defined, even though functional activation of fibroblasts has been suggested. The study was aimed to determine the effects of glycolic acid and malic acid (AHA: alpha hydroxy acid) on cultured dermal fibroblasts. Whether it directly increases cell proliferation may be an important factor influencing the production of extracellular matrix such as type I collagen. Cultured human skin fibroblasts were treated for 24 hours with glycolic acid and malic acid at different concentrations (10(-4), 10(-5), 10(-6) M), and cell proliferation was measured by MTT assay. Then quantitative analysis of collagen synthesis was performed by PICP (Procollagen Type I C-peptide) enzyme immunoassay and radioisotope (3H-proline) labelled collagen assay. The results showed increased cell proliferation and collagen production in response to glycolic acid in a dose dependent manner. The range of cell proliferation and collagen production were significantly higher with glycolic acid treatment than with malic acid or control. It was suggested that the favorable effects of glycolic acid treatment on aging skin were mediated by increased cell proliferation in addition to functional activation of fibroblasts.
已知乙醇酸焕肤可改善诸如皱纹和粗糙等光老化问题，尽管已有研究表明其可激活成纤维细胞的功能，但这种效果尚未得到明确界定。本研究旨在确定乙醇酸和苹果酸（α-羟基酸）对培养的真皮成纤维细胞的影响。它是否直接增加细胞增殖可能是影响细胞外基质如I型胶原蛋白产生的一个重要因素。用不同浓度（10⁻⁴、10⁻⁵、10⁻⁶ M）的乙醇酸和苹果酸对培养的人皮肤成纤维细胞处理24小时，通过MTT法检测细胞增殖。然后通过I型前胶原C肽（PICP）酶免疫测定法和放射性同位素（³H-脯氨酸）标记的胶原蛋白测定法对胶原蛋白合成进行定量分析。结果显示，乙醇酸可使细胞增殖和胶原蛋白生成呈剂量依赖性增加。乙醇酸处理组的细胞增殖和胶原蛋白生成范围显著高于苹果酸处理组或对照组。研究表明，乙醇酸处理对老化皮肤的有益作用除了激活成纤维细胞功能外，还通过增加细胞增殖来介导。
