Kim S J, Park J H, Kim D H, Won Y H, Maibach H I
Department of Dermatology, Chonnam University Research Institute of Medical Science, Chonnam National University Medical School, Kwangju, Korea.
Dermatol Surg. 1998 Oct;24(10):1054-8. doi: 10.1111/j.1524-4725.1998.tb04074.x.
Glycolic acid treatment is believed to reverse the photoaging process by increasing collagen synthesis in the skin. However, this effect has not been clearly defined even though alpha hydroxy acid products are used extensively.
This study aimed to define the primary effect of glycolic acid on collagen synthesis that may be achieved by functional activation or proliferation of fibroblasts.
Glycolic acid treatment was compared in vivo with lactic acid (hairless mice) and in vitro to malic acid (normal human skin fibroblast culture) with controls. To find the functional activation of fibroblasts, Northern blot assay for type I collagen synthesis with histometric analysis (in vivo) was performed. Cell proliferation assay (MTT) with procollagen type I C-peptide (PICP) enzyme immunoassay and radioisotope ([3H]proline) incorporated collagen production from cultured fibroblasts were determined.
The in vivo collagen mRNA expression with histometric analysis revealed greater collagen synthesis by glycolic acid compared with lactic acid and control. In vitro cell proliferative effect of glycolic and greater amount of collagen production showed a steady increase in a dose-dependent manner.
Both in vivo and in vitro, glycolic acid treatment increased the production of collagen and fibroblast proliferation. These effects may be the mechanism by which glycolic acid reverses the process of photoaging.
乙醇酸治疗被认为可通过增加皮肤中的胶原蛋白合成来逆转光老化过程。然而，尽管α - 羟基酸产品被广泛使用，但这种效果尚未明确界定。
本研究旨在确定乙醇酸对胶原蛋白合成的主要作用，这可能是通过成纤维细胞的功能激活或增殖来实现的。
将乙醇酸治疗在体内与乳酸（无毛小鼠）进行比较，并在体外与苹果酸（正常人皮肤成纤维细胞培养）及对照组进行比较。为了发现成纤维细胞的功能激活，进行了用于I型胶原蛋白合成的Northern印迹分析以及组织测量分析（体内）。测定了细胞增殖试验（MTT）、I型前胶原C肽（PICP）酶免疫测定以及来自培养成纤维细胞的放射性同位素（[3H]脯氨酸）掺入的胶原蛋白生成量。
通过组织测量分析的体内胶原蛋白mRNA表达显示，与乳酸和对照组相比，乙醇酸可促进更多的胶原蛋白合成。体外，乙醇酸的细胞增殖作用以及更多的胶原蛋白生成量呈剂量依赖性稳步增加。
在体内和体外，乙醇酸治疗均增加了胶原蛋白的生成和成纤维细胞的增殖。这些作用可能是乙醇酸逆转光老化过程的机制。
