Donia Abd-El-Raheim Mohammed, Soliman Gamal Abd-El-Hakim, El-Sakhawy Mohamed Abd-El-Monem, Yusufoglu Hasan, Zaghloul Ahmed Mohamed
Pharmacognosy Department, College of Pharmacy, Salman Bin Abdulaziz University, Al-Kharj, KSA / Medicinal and Aromatic Plants Department, Desert Research Center, Cairo, Egypt.
Pharmacology Department, College of Pharmacy, Salman Bin Abdulaziz University, Al-Kharj, KSA / Pharmacology Department, College of Veterinary Medicine, Cairo University, Egypt.
Pak J Pharm Sci. 2014 Mar;27(2):351-6.
The current research was designed to evaluate the phytochemical contents, cytotoxic and antimicrobial activity of Emex spinosa extracts. The different plant extracts and Aloe-emodin glucoside were screened using the colorimetric MTT method (3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyl- tetrazolium bromide) assay to test their in vitro cytotoxic activity against HepG2, MCF-7, Caco-2 and HCT. The clinically used anticancer drug doxorubicin was used as standard for comparative purposes. Anthraquinones (Aloe-emodin-O-glucoside, Emodin and nataloin (1, 2, 8-trihydroxy, 6-methyl, 10-anthrone-C-glucoside) together with β-sistosterol and β-sitisterol-O-β-D-glucoside were isolated from Emex spinosa. Aloe-emodin glucoside together with four fractions from this plant were evaluated for their anticancer and antimicrobial activities. Aloe-emodin glucoside showed anticancer activity against HCT, HepG-2, MCF-7 and Caco-2 cell lines. The total ethanol extract of E. spinosa and diethyl ether, chloroform, ethyl acetate and butanol fractions shown antibacterial activity against Staphylococcus aureus, Streptococcus pyogenes and Bacillus subtilis.
本研究旨在评估刺果沙蓬提取物的植物化学成分、细胞毒性和抗菌活性。采用比色法MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑）试验对不同植物提取物和芦荟大黄素葡萄糖苷进行筛选，以测试它们对肝癌细胞系HepG2、乳腺癌细胞系MCF-7、结肠癌细胞系Caco-2和人结肠癌细胞系HCT的体外细胞毒性活性。临床使用的抗癌药物阿霉素用作比较标准。从刺果沙蓬中分离出蒽醌类化合物（芦荟大黄素-O-葡萄糖苷、大黄素和萘塔林（1,2,8-三羟基、6-甲基、10-蒽酮-C-葡萄糖苷）以及β-谷甾醇和β-谷甾醇-O-β-D-葡萄糖苷。对芦荟大黄素葡萄糖苷及其该植物的四个馏分进行了抗癌和抗菌活性评估。芦荟大黄素葡萄糖苷对人结肠癌细胞系HCT、肝癌细胞系HepG-2、乳腺癌细胞系MCF-7和结肠癌细胞系Caco-2显示出抗癌活性。刺果沙蓬的总乙醇提取物以及二乙醚、氯仿、乙酸乙酯和丁醇馏分对金黄色葡萄球菌、化脓性链球菌和枯草芽孢杆菌显示出抗菌活性。
