QmnD3/QmnD4 在 quartromicin 生物合成中用于形成双键的特性。

Characterization of QmnD3/QmnD4 for double bond formation in quartromicin biosynthesis.

机构信息

State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.

出版信息

Org Lett. 2014 Mar 21;16(6):1578-81. doi: 10.1021/ol500111n. Epub 2014 Feb 28.

DOI:10.1021/ol500111n

PMID:24580034

Abstract

In this work, two enzymes responsible for the biogenesis of possible [4 + 2] reaction precursors in the quartromicin biosynthetic pathway were characterized: acetylation of 1 to yield 2 was catalyzed by QmnD3, and subsequent acetic acid elimination of 2 to form double bond product 3 was catalyzed by QmnD4. Site-directed mutagenesis assay of QmnD3 and QmnD4 was investigated, and a general base-catalyzed mechanism for QmnD4 is proposed.

摘要

在这项工作中，我们对参与 quartromicin 生物合成途径中 [4 + 2] 反应前体生物合成的两种酶进行了表征：QmnD3 催化 1 的乙酰化生成 2，而 QmnD4 则催化 2 的乙酸消除形成双键产物 3。我们还对 QmnD3 和 QmnD4 进行了定点突变实验，并提出了 QmnD4 的广义碱催化机制。