Pesce Giuseppe, Rusciano Giulia, Sasso Antonio, Isticato Rachele, Sirec Teja, Ricca Ezio
Dipartimento di Fisica Università degli studi di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 80126, Napoli, Italy.
Dipartimento di Fisica Università degli studi di Napoli, Complesso Universitario Monte S. Angelo, Via Cintia 80126, Napoli, Italy.
Colloids Surf B Biointerfaces. 2014 Apr 1;116:568-75. doi: 10.1016/j.colsurfb.2014.01.039. Epub 2014 Feb 4.
In this work we report on the simultaneous measurement of the hydrodynamic coefficient and the electric charge of single Bacillus subtilis spores. The latter has great importance in protein binding to spores and in the adhesion of spores onto surfaces. The charge and the hydrodynamic coefficient were measured by an accurate procedure based on the analysis of the motion of single spores confined by an optical trap. The technique has been validated using charged spherical polystyrene beads. The excellent agreement of our results with the expected values demonstrates the quality of our procedure. We measured the charge of spores of B. subtilis purified from a wild type strain and from two isogenic mutants characterized by an altered spore surface. Our technique is able to discriminate the three spore types used, by their charge and by their hydrodynamic coefficient which is related to the hydrophobic properties of the spore surface.
在这项工作中，我们报告了对单个枯草芽孢杆菌孢子的流体动力学系数和电荷的同时测量。后者在蛋白质与孢子的结合以及孢子在表面的黏附中具有重要意义。电荷和流体动力学系数通过基于对由光阱限制的单个孢子运动分析的精确程序进行测量。该技术已使用带电的球形聚苯乙烯珠进行了验证。我们的结果与预期值的出色吻合证明了我们程序的质量。我们测量了从野生型菌株以及两个具有改变的孢子表面特征的同基因突变体中纯化的枯草芽孢杆菌孢子的电荷。我们的技术能够通过所使用的三种孢子类型的电荷以及与孢子表面疏水特性相关的流体动力学系数来区分它们。
