Sirec Teja, Benarroch Jonatan M, Buffard Pauline, Garcia-Ojalvo Jordi, Asally Munehiro
School of Life Sciences, The University of Warwick, Coventry CV4 7AL, UK.
School of Life Sciences, The University of Warwick, Coventry CV4 7AL, UK; Warwick Medical School, The University of Warwick, Coventry CV4 7AL, UK.
iScience. 2019 Jun 28;16:378-389. doi: 10.1016/j.isci.2019.05.044. Epub 2019 Jun 5.
Quality control of offspring is important for the survival of cells. However, the mechanisms by which quality of offspring cells may be checked while running genetic programs of cellular differentiation remain unclear. Here we investigated quality control during sporulating in Bacillus subtilis by combining single-cell time-lapse microscopy, molecular biology, and mathematical modeling. Our results revealed that the quality control via premature germination is coupled with the electrical polarization of outer membranes of developing forespores. The forespores that accumulate fewer cations on their surface are more likely to be aborted. This charge accumulation enables the projection of multi-dimensional information about the external environment and morphological development of the forespore into one-dimensional information of cation accumulation. We thus present a paradigm of cellular regulation by bacterial electrical signaling. Moreover, based on the insight we gain, we propose an electrophysiology-based approach of reducing the yield and quality of Bacillus endospores.
子代细胞的质量控制对细胞存活至关重要。然而，在运行细胞分化的遗传程序时，检查子代细胞质量的机制仍不清楚。在这里，我们通过结合单细胞延时显微镜、分子生物学和数学建模，研究了枯草芽孢杆菌孢子形成过程中的质量控制。我们的结果表明，通过过早萌发进行的质量控制与发育中的前芽孢外膜的电极化相关联。表面积累较少阳离子的前芽孢更有可能夭折。这种电荷积累能够将关于外部环境和前芽孢形态发育的多维信息投射到阳离子积累的一维信息中。因此，我们提出了一种细菌电信号介导的细胞调节模式。此外，基于我们获得的见解，我们提出了一种基于电生理学的方法来降低枯草芽孢杆菌芽孢的产量和质量。
