Englyst H N, Quigley M E, Hudson G J, Cummings J H
Medical Research Council Dunn Clinical Nutrition Centre, Cambridge, UK.
Analyst. 1992 Nov;117(11):1707-14. doi: 10.1039/an9921701707.
An improved method is described for the measurement of total, soluble and insoluble dietary fibre as non-starch polysaccharides (NSP). An established procedure is modified to allow more rapid removal of starch and hydrolysis of NSP. In its present form the procedure is simpler and more robust than those previously published. In the modified method starch is removed enzymically within 50 min and NSP is precipitated with ethanol and then hydrolysed by treatment with sulfuric acid for 2 h. The constituent sugars can in turn be measured by gas-liquid chromatography, high-performance liquid chromatography or more rapidly by colorimetry. The improved procedure described here for the removal of starch and hydrolysis of NSP applies to all three techniques, but only the method for measurement of sugars by gas-liquid chromatography is described here in full.
本文描述了一种改进的方法，用于测定作为非淀粉多糖（NSP）的总膳食纤维、可溶性膳食纤维和不溶性膳食纤维。对既定程序进行了修改，以便更快速地去除淀粉并水解NSP。以目前的形式，该程序比以前发表的程序更简单、更可靠。在改进后的方法中，淀粉在50分钟内通过酶法去除，NSP用乙醇沉淀，然后用硫酸处理2小时进行水解。组成糖依次可通过气液色谱法、高效液相色谱法或更快速地通过比色法进行测定。本文所述的去除淀粉和水解NSP的改进程序适用于所有这三种技术，但这里仅完整描述了通过气液色谱法测定糖的方法。
