Englyst H N, Quigley M E, Hudson G J
Dunn Clinical Nutrition Centre, Addenbrookes Hospital Site, Cambridge, UK.
Analyst. 1994 Jul;119(7):1497-509. doi: 10.1039/an9941901497.
Methods for the measurement of dietary fibre as non-starch polysaccharides (NSP) are described. A common enzymic removal of starch and acid hydrolysis of the NSP to their constituent sugars are followed by one of three alternative techniques, gas-liquid chromatography, high-performance liquid chromatography or spectrophotometry, for measurement of the released sugars. The results obtained by the three methods are in good agreement for a wide range of raw and processed foods. NSP compose approximately 90% of the plant cell-wall material and are therefore a good index of this material. Values for NSP therefore provide a good marker for a diet rich in fruit, vegetables and high-extraction cereal products associated with health and recommended in dietary guidelines. Values for total, soluble and insoluble NSP may be obtained with any of the end-point techniques, and the detailed information obtained from the chromatographic methods is useful in studies of the relationship between the intakes of various types of NSP and health. The causes of some potential interferences in the spectrophotometric assay, especially from processed foods, have been identified and eliminated. The rapid spectrophotometric version is suitable for food labelling purposes and for quality control, and the changes described have made it more robust.
本文描述了将膳食纤维作为非淀粉多糖（NSP）进行测量的方法。通常先通过酶法去除淀粉，然后将NSP酸水解为其组成糖类，接着采用三种替代技术之一，即气液色谱法、高效液相色谱法或分光光度法，来测量释放出的糖类。对于多种生的和加工食品，这三种方法所获得的结果高度一致。NSP约占植物细胞壁物质的90%，因此是这种物质的良好指标。所以，NSP的值可为富含水果、蔬菜和高提取率谷物产品的饮食提供一个良好的标志，这类饮食有益健康且在饮食指南中被推荐。通过任何一种终点技术都可以获得总NSP、可溶性NSP和不溶性NSP的值，并且从色谱方法中获得的详细信息对于研究各类NSP的摄入量与健康之间的关系很有用。已经识别并消除了分光光度法测定中一些潜在干扰的原因，特别是来自加工食品的干扰。快速分光光度法适用于食品标签目的和质量控制，并且所描述的改进使其更加可靠。
