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添加蛋白酶的饲用豌豆对肉鸡的可代谢能量及可消化养分值

Metabolizable energy and digestible nutrient values of protease-supplemented field pea for broiler chicken.

作者信息

Woyengo Tofuko A, Rogiewicz Anna, Patterson Robert

机构信息

Department of Animal and Veterinary Sciences, Aarhus University, Blichers Allé 20, DK-8830 Tjele, Denmark.

Department of Animal Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.

出版信息

Poult Sci. 2025 Feb;104(2):104675. doi: 10.1016/j.psj.2024.104675. Epub 2024 Dec 17.

Abstract

A study was conducted to determine the effects of protease supplementation of field pea (in comparison with soybean meal; SBM) for broilers on apparent metabolizable energy (AMEn) and standardized ileal digestibility (SID) of amino acids (AA). One hundred and forty broiler chicks were divided into 35 groups of 4 birds/group and fed 5 diets in a completely randomized design (7 groups/diet) from 14 to 21 d of age. The diets were cornstarch-based containing SBM or field pea as the sole protein source without or with protease (ProSparity 250; CBS Bio Platforms, Calgary, AB, Canada) in 2 × 2 factorial arrangement, and N-free diet. Protease was added to diets to supply 6,250 U/kg. Digestibility of AA and N retention for feedstuffs were determined by the direct method, whereas digestibility and retention of energy for feedstuffs were determined by the difference from the N-free diet. On as fed basis, SBM and field pea contained 90.0 and 88.9 dry matter, 46.6 and 20.2 % crude protein, 2.88 and 1.47 % Lys, and 14.3 and 14.7 % non-starch polysaccharides, respectively. On as fed basis, the AMEn value for field pea was lower (P < 0.05) than that of SBM (2,006 vs. 2,414 kcal/kg). Also, the SID values of most indispensable AA in field pea were lower (P < 0.05) than those in SBM. Feedstuff (field pea vs. SBM) and protease did not interact on AMEn and SID of AA values. The supplemental protease increased (P < 0.05) AMEn values of the SBM and field by an average of 187 kcal/kg but decreased (P < 0.05) the SID values of all indispensable AA (except His) for the SBM and field, by an average of 2.7 percentage points. Results indicate that the protease product used in the current study marginally reduced the SID of AA for both SBM and field pea. However, the protease product can be beneficially included in diets based on SBM or field pea for broilers to enhance their AMEn values. The response to the protease on the AMEn was consistent regardless of the protein source in the diet, implying that the protease used in this study has similar positive impact on energy availability in both field pea and SBM.

摘要

本研究旨在确定添加蛋白酶对肉仔鸡饲用豌豆(与豆粕相比)的表观代谢能(AMEn)和氨基酸(AA)的标准回肠消化率(SID)的影响。140只肉仔鸡雏鸡被分为35组,每组4只,并在14至21日龄期间采用完全随机设计(每种日粮7组)饲喂5种日粮。日粮以玉米淀粉为基础,含有豆粕或豌豆作为唯一蛋白质来源,采用2×2析因设计,分别添加或不添加蛋白酶(ProSparity 250;CBS生物平台,加拿大阿尔伯塔省卡尔加里),以及无氮日粮。向日粮中添加蛋白酶,使其含量达到6250 U/kg。饲料中氨基酸的消化率和氮保留率采用直接法测定,而饲料中能量的消化率和保留率则通过与无氮日粮的差值来测定。以饲喂状态计,豆粕和豌豆的干物质含量分别为90.0%和88.9%,粗蛋白含量分别为46.6%和20.2%,赖氨酸含量分别为2.88%和1.47%,非淀粉多糖含量分别为14.3%和14.7%。以饲喂状态计,豌豆的AMEn值低于豆粕(P<0.05)(分别为2006和2414 kcal/kg)。此外,豌豆中大多数必需氨基酸的SID值低于豆粕(P<0.05)。饲料原料(豌豆与豆粕)和蛋白酶对AA值的AMEn和SID没有交互作用。添加的蛋白酶使豆粕和豌豆的AMEn值平均提高了187 kcal/kg(P<0.05),但使豆粕和豌豆中所有必需氨基酸(组氨酸除外)的SID值平均降低了2.7个百分点(P<0.05)。结果表明,本研究中使用的蛋白酶产品略微降低了豆粕和豌豆中氨基酸的SID。然而,蛋白酶产品可有益地添加到以豆粕或豌豆为基础的肉仔鸡日粮中,以提高其AMEn值。无论日粮中的蛋白质来源如何,蛋白酶对AMEn的反应都是一致的,这意味着本研究中使用的蛋白酶对豌豆和豆粕中的能量利用率具有相似的积极影响。

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