Chemistry Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.
Chemistry Department, Faculty of Science, Benha University, Benha, Egypt.
J Anal Methods Chem. 2014;2014:286379. doi: 10.1155/2014/286379. Epub 2014 Jan 22.
SIMPLE, RAPID, AND EXTRACTIVE SPECTROPHOTOMETRIC METHODS WERE DEVELOPED FOR THE DETERMINATION OF SOME FLUOROQUINOLONES ANTIBIOTICS: gemifloxacin mesylate (GMF), moxifloxacin hydrochloride (MXF), and enrofloxacin (ENF) in pure forms and pharmaceutical formulations. These methods are based on the formation of ion-pair complexes between the basic drugs and acid dyes, namely, bromocresol green (BCG), bromocresol purple (BCP), bromophenol blue (BPB), bromothymol blue (BTB), and methyl orange (MO) in acidic buffer solutions. The formed complexes were extracted with chloroform and measured at 420, 408, 416, 415, and 422 nm for BCG, BCP, BPB, BTB, and MO, respectively, for GMF; at 410, 415, 416, and 420 nm for BCP, BTB, BPB, and MO, respectively, for MXF; and at 419 and 414 nm for BCG and BTB, respectively, in case of ENF. The analytical parameters and their effects are investigated. Beer's law was obeyed in the ranges 1.0-30, 1.0-20, and 2.0-24 μ g mL(-1) for GMF, MXF, and ENF, respectively. The proposed methods have been applied successfully for the analysis of the studied drugs in pure forms and pharmaceutical formulations. Statistical comparison of the results with the reference methods showed excellent agreement and indicated no significant difference in accuracy and precision.
甲磺酸加替沙星（GMF）、盐酸莫西沙星（MXF）和恩诺沙星（ENF）的纯品和药物制剂的简单、快速和萃取分光光度法。这些方法基于碱性药物与酸性染料（溴甲酚绿（BCG）、溴甲酚紫（BCP）、溴酚蓝（BPB）、溴百里酚蓝（BTB）和甲基橙（MO））在酸性缓冲溶液中形成离子对配合物。形成的配合物用氯仿萃取，分别在 420、408、416、415 和 422nm 处测定 BCG、BCP、BPB、BTB 和 MO 用于 GMF；在 410、415、416 和 420nm 处测定 BCP、BTB、BPB 和 MO 用于 MXF；在 419 和 414nm 处测定 BCG 和 BTB 用于 ENF。研究了分析参数及其影响。GMF、MXF 和 ENF 的比尔定律线性范围分别为 1.0-30、1.0-20 和 2.0-24μg mL(-1)。该方法已成功应用于纯品和药物制剂中研究药物的分析。与参考方法的结果统计比较表明，准确性和精密度无显著差异，具有良好的一致性。
