Radi Abd-Elgawad, Khafagy Amira, El-Shobaky Amira, El-Mezayen Hatem
Department of Chemistry, Faculty of Science, Dumyat University; 34517 Dumyat, Egypt.
Department of Chemistry, Faculty of Science, Helwan University, 11795 Helwan, Egypt.
J Pharm Anal. 2013 Apr;3(2):132-136. doi: 10.1016/j.jpha.2012.10.005. Epub 2012 Nov 2.
The electrochemical oxidation behavior and voltammetric assay of gemifloxacin were investigated using differential-pulse and cyclic voltammetry on a screen-printed carbon electrode. The effects of pH, scan rates, and concentration of the drug on the anodic peak current were studied. Voltammograms of gemifloxacin in Tris-HCl buffer (pH 7.0) exhibited a well-defined single oxidation peak. A differential-pulse voltammetric procedure for the quantitation of gemifloxacin has been developed and suitably validated with respect to linearity, limits of detection and quantification, accuracy, precision, specificity, and robustness. The calibration was linear from 0.5 to 10.0 μM, and the limits of detection and quantification were 0.15 and 5.0 μM. Recoveries ranging from 96.26% to 103.64% were obtained. The method was successfully applied to the determination of gemifloxacin in pharmaceutical tablets without any pre-treatment. Excipients present in the tablets did not interfere in the assay.
采用差分脉冲伏安法和循环伏安法，在丝网印刷碳电极上研究了吉米沙星的电化学氧化行为和伏安测定法。研究了pH值、扫描速率和药物浓度对阳极峰电流的影响。吉米沙星在Tris-HCl缓冲液（pH 7.0）中的伏安图显示出一个明确的单一氧化峰。已开发出一种用于定量吉米沙星的差分脉冲伏安法，并在线性、检测限和定量限、准确度、精密度、特异性和稳健性方面进行了适当验证。校准在0.5至10.0 μM范围内呈线性，检测限和定量限分别为0.15和5.0 μM。回收率在96.26%至103.64%之间。该方法成功应用于药物片剂中吉米沙星的测定，无需任何预处理。片剂中的辅料不干扰测定。
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