Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Biomed Res Int. 2014;2014:792086. doi: 10.1155/2014/792086. Epub 2014 Jan 22.
Wound healing involves inflammation followed by granular tissue development and scar formation. In this study, synthetic chalcone 3-(2-Chlorophenyl)-1-phenyl-propenone (CPPP) was investigated for a potential role in enhancing wound healing and closure. Twenty-four male rats were divided randomly into 4 groups: carboxymethyl cellulose (CMC) (0.2 mL), Intrasite gel, and CPPP (25 or 50 mg/mL). Gross morphology, wounds treatment with the CPPP, and Intrasite gel accelerate the rate of wound healing compared to CMC group. Ten days after surgery, the animals were sacrificed. Histological assessment revealed that the wounds treated with CPPP showed that wound closure site contained little amount of scar and the granulation tissue contained more collagen and less inflammatory cells than wound treated with CMC. This finding was confirmed with Masson's trichrome staining. The antioxidant defence enzymes catalase (CAT) and superoxide dismutase (SOD) were significantly increased in the wound homogenates treated with CPPP (P < 0.05) compared to CMC treated group. However, in the CPPP treatment group, lipid peroxidation (MDA) was significantly decreased (P < 0.05), suggesting that the CPPP also has an important role in protection against lipid peroxidation-induced skin injury after ten days of treatment with CPPP, which is similar to the values of cytokines TGF-β and TNF-α in tissue homogenate. Finally the administration of CPPP at a dosage of 25 and 50 mg/kg was suitable for the stimulation of wound healing.
伤口愈合包括炎症反应，随后是颗粒组织的形成和瘢痕形成。在这项研究中，研究了合成查尔酮 3-(2-氯苯基)-1-苯基-丙烯酮（CPPP）在促进伤口愈合和闭合中的潜在作用。24 只雄性大鼠随机分为 4 组：羧甲基纤维素（CMC）（0.2 mL）、Instrasite 凝胶和 CPPP（25 或 50 mg/mL）。大体形态学观察、CPPP 和 Instrasite 凝胶处理伤口均能加速伤口愈合，与 CMC 组相比。手术后 10 天，处死动物。组织学评估显示，用 CPPP 处理的伤口，伤口闭合部位的瘢痕组织较少，肉芽组织中含有较多的胶原蛋白，炎症细胞较少，而用 CMC 处理的伤口则相反。Masson 三色染色证实了这一发现。与 CMC 处理组相比，用 CPPP 处理的伤口匀浆中抗氧化防御酶过氧化氢酶（CAT）和超氧化物歧化酶（SOD）显著增加（P <0.05）。然而，在 CPPP 处理组中，脂质过氧化（MDA）显著降低（P <0.05），这表明 CPPP 还在保护脂质过氧化引起的皮肤损伤方面发挥着重要作用，这与组织匀浆中细胞因子 TGF-β和 TNF-α的数值相似。最后，CPPP 以 25 和 50mg/kg 的剂量给药适合刺激伤口愈合。
