Biomolecular Research Group, Biochemistry Program, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Department of Pharmacology, Faculty of Medicine, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Int J Surg. 2015 Jun;18:110-7. doi: 10.1016/j.ijsu.2015.03.026. Epub 2015 Apr 18.
Annona muricata, a member of the Annonaceae family, is commonly known as soursop and graviola. The leaves of this tropical fruit tree are widely used in folk medicine against skin diseases and abscesses, however there is no scientific evidence justifying the use of A. muricata leaves. The aim of the present study is to evaluate the wound healing potential of ethyl acetate extract of A. muricata leaves (EEAM) towards excisional wound models in rats.
Sprague Dawley rats (24) were randomly divided into four groups, viz. (A) vehicle control, (B) low dose of EEAM (5% w/w), (C) high dose of EEAM (10% w/w) and (D) positive control with excisional wound created on the neck area. Wounds were topically dressed twice a day for 15 days. On the 15th day, animals were sacrificed and then processed for immunohistochemical and histological evaluations, including Hematoxylin & Eosin and Masson Trichrome stainings. The activity of antioxidants, namely catalase, glutathione peroxidase and superoxide dismutase, and malondialdehyde (MDA) was measured in wound tissue homogenate.
Macroscopic and microscopic analysis of wounds demonstrated a significant wound healing activity shown by EEAM at two doses. Treatment of wounds with ointment containing EEAM caused significant surge in antioxidants activities and decrease in the MDA level of wound tissues compared with vehicle control. The immunohistochemical evaluation revealed conspicuous up-regulation of Hsp70 in treated wounds with EEAM, suggesting the anti-inflammatory effect of EEAM.
EEAM exhibited a promising wound healing potential towards excisional wound models in rats.
番荔枝，番荔枝科的一员，俗称刺果番荔枝和巴西番荔枝。这种热带果树的叶子在民间医学中被广泛用于治疗皮肤病和脓肿，但没有科学证据证明番荔枝叶的使用是合理的。本研究旨在评估番荔枝叶乙酸乙酯提取物（EEAM）对大鼠切除伤口模型的愈合潜力。
将 24 只 Sprague Dawley 大鼠随机分为四组，即（A）赋形剂对照组、（B）EEAM 低剂量组（5%w/w）、（C）EEAM 高剂量组（10%w/w）和（D）用颈部区域的切除伤口作为阳性对照。伤口每天用药物处理两次，共 15 天。在第 15 天，处死动物，然后进行免疫组织化学和组织学评估，包括苏木精和伊红（H&E）和马松三色（Masson Trichrome）染色。测量伤口组织匀浆中的抗氧化剂活性，如过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶，以及丙二醛（MDA）。
宏观和微观分析表明，EEAM 两种剂量均表现出显著的伤口愈合活性。与赋形剂对照组相比，用含有 EEAM 的软膏处理伤口会导致抗氧化剂活性显著增加，MDA 水平降低。免疫组织化学评价显示，EEAM 处理的伤口中 Hsp70 的表达明显上调，提示 EEAM 具有抗炎作用。
EEAM 对大鼠切除伤口模型表现出有希望的愈合潜力。
