Rouhollahi Elham, Moghadamtousi Soheil Zorofchian, Hajiaghaalipour Fatemeh, Zahedifard Maryam, Tayeby Faezeh, Awang Khalijah, Abdulla Mahmood Ameen, Mohamed Zahurin
Pharmacogenomics Laboratory, Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Drug Des Devel Ther. 2015 Oct 27;9:5805-13. doi: 10.2147/DDDT.S88196. eCollection 2015.
Curcuma purpurascens BI. is a member of Zingiberaceae family. The purpose of this study is to investigate the wound healing properties of hexane extract of C. purpurascens rhizome (HECP) against excisional wound healing in rats.
Twenty four rats were randomly divided into 4 groups: A) negative control (blank placebo, acacia gum), B) low dose of HECP, C) high dose of HECP, and D) positive control, with 6 rats in each group. Full-thickness incisions (approximately 2.00 cm) were made on the neck area of each rat. Groups 1-4 were treated two-times a day for 20 days with blank placebo, HECP (100 mg/kg), HECP (200 mg/kg), and intrasite gel as a positive control, respectively. After 20 days, hematoxylin and eosin and Masson's trichrome stainings were employed to investigate the histopathological alterations. Protein expressions of Bax and Hsp70 were examined in the wound tissues using immunohistochemistry analysis. In addition, levels of enzymatic antioxidants and malondialdehyde representing lipid peroxidation were measured in wound tissue homogenates.
Macroscopic evaluation of wounds showed conspicuous elevation in wound contraction after topical administration of HECP at both doses. Moreover, histopathological analysis revealed noteworthy reduction in the scar width correlated with the enhanced collagen content and fibroblast cells, accompanied by a reduction of inflammatory cells in the granulation tissues. At the molecular level, HECP facilitates wound-healing process by downregulating Bax and upregulating Hsp70 protein at the wound site. The formation of new blood vessel was observed in Masson's trichrome staining of wounds treated with HECP (100 and 200 mg/kg). In addition, HECP administration caused a significant surge in enzymatic antioxidant activities and a decline in lipid peroxidation.
These findings suggested that HECP accelerated wound-healing process in rats via antioxidant activity, angiogenesis effect and anti-inflammatory responses involving Hsp70/Bax.
紫姜黄是姜科植物的一种。本研究旨在探讨紫姜黄根茎己烷提取物（HECP）对大鼠切除伤口愈合的促进作用。
将24只大鼠随机分为4组：A）阴性对照组（空白安慰剂，阿拉伯树胶），B）低剂量HECP组，C）高剂量HECP组，D）阳性对照组，每组6只。在每只大鼠的颈部区域制作全层切口（约2.00 cm）。第1 - 4组分别用空白安慰剂、HECP（100 mg/kg）、HECP（200 mg/kg）和创面用凝胶作为阳性对照，每天处理两次，持续20天。20天后，采用苏木精 - 伊红染色和Masson三色染色法研究组织病理学改变。采用免疫组织化学分析法检测伤口组织中Bax和Hsp70的蛋白表达。此外，测定伤口组织匀浆中代表脂质过氧化的酶促抗氧化剂和丙二醛水平。
伤口的宏观评估显示，两种剂量的HECP局部给药后伤口收缩明显增加。此外，组织病理学分析显示，瘢痕宽度显著减小，与胶原蛋白含量增加和成纤维细胞增多相关，同时肉芽组织中的炎症细胞减少。在分子水平上，HECP通过下调伤口部位的Bax和上调Hsp70蛋白促进伤口愈合过程。在HECP（100和200 mg/kg）处理的伤口的Masson三色染色中观察到新血管形成。此外，HECP给药导致酶促抗氧化活性显著增加，脂质过氧化水平下降。
这些结果表明，HECP通过抗氧化活性、血管生成作用以及涉及Hsp70/Bax的抗炎反应加速大鼠伤口愈合过程。
