Siddiqi Nikhat J, Zargar Seema
Department of Biochemistry, College of Science, King Saud University , Riyadh , Saudi Arabia.
Connect Tissue Res. 2014 Jun;55(3):234-8. doi: 10.3109/03008207.2014.900551. Epub 2014 Apr 1.
PURPOSE/AIM OF THE STUDY: To study the effect of quercetin, a flavanoid on cadmium fluoride-induced alterations in hydroxyproline and collagen content in mice liver.
Following experimental groups were studied: Group 1, normal mice; Group 2, CdF2-treated mice administered single intraperitoneal (i.p.) injections of CdF2 1 mg/kg bw (body weight); Group 3, CdF2-treated mice administered single i.p. injections of CdF2 2 mg/kg bw; Group 4, mice-injected i.p. with 100 mg quercetin/kg bw; and Group 5, mice-injected i.p. with 100 mg quercetin/kg bw followed by 2 mg CdF2/kg bw after 2 h. Mice were sacrificed 24 h after CdF injection by asphyxiation with carbon dioxide.
1 mg/kg and 2 mg/kg body weight (bw) of CdF2 caused a significant increase in hepatic total Hyp and collagen when compared with the liver of control mice. This was associated with significant changes in free, peptide bound, and protein bound Hyp fraction in the livers of treated mice. Quercetin treatment alone and with CdF2 also caused a significant increase in total Hyp and total collagen in mice liver.
We conclude that quercetin has a synergestic effect with CdF2 on the total Hyp and collagen content in mice liver.
研究类黄酮槲皮素对氟化镉诱导的小鼠肝脏羟脯氨酸和胶原蛋白含量变化的影响。
研究了以下实验组：第1组，正常小鼠；第2组，经腹腔注射1mg/kg体重氟化镉（CdF2）处理的小鼠；第3组，经腹腔注射2mg/kg体重CdF2处理的小鼠；第4组，经腹腔注射100mg/kg体重槲皮素的小鼠；第5组，经腹腔注射100mg/kg体重槲皮素，2小时后再经腹腔注射2mg/kg体重CdF2的小鼠。注射CdF2后24小时，通过二氧化碳窒息法处死小鼠。
与对照小鼠肝脏相比，1mg/kg和2mg/kg体重的CdF2导致肝脏总羟脯氨酸（Hyp）和胶原蛋白显著增加。这与处理组小鼠肝脏中游离、肽结合和蛋白质结合的Hyp组分的显著变化相关。单独使用槲皮素处理以及与CdF2联合处理也导致小鼠肝脏中总Hyp和总胶原蛋白显著增加。
我们得出结论，槲皮素与CdF2对小鼠肝脏中总Hyp和胶原蛋白含量具有协同作用。
