1 Biochemistry Department, College of Sciences, King Saud University, Riyadh, Saudi Arabia.
2 Pharmaceutical Chemistry Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Int J Immunopathol Pharmacol. 2017 Sep;30(3):207-214. doi: 10.1177/0394632017714175. Epub 2017 Jun 7.
This study was designed to evaluate the effect of rutin on hepatotoxicity induced by thioacetamide (TAA) in rats. Four groups of male Wistar rats consisting of six rats each were used: Group I: control group; Group II: rats receiving single injection of 300 mg kg body weight of TAA intraperitoneally; Group III: rats administered rutin (10 mg kg body weight) dissolved in saline orally for 2 weeks; and Group IV: rats administered rutin (10 mg kg body weight) dissolved in saline orally for 2 weeks followed by TAA injection last day of second week. All groups were sacrificed after 24 h of treatment and hepatic toxicity was analyzed with respect to liver toxicity markers, liver DNA fragmentation, and histology of liver tissue. Administration of TAA in Wistar rats resulted in significant increase of hepatic markers, DNA fragmentation in the hepatocytes, and changes in histology. Pretreatment of rats with rutin before 2 weeks of TAA assault resulted in the complete reversal of TAA-mediated hepatic toxicity ( P < 0.0001 to P < 0.01) with concomitant restoration of DNA fragmentation. This study suggests rutin as a protective agent for restoration of toxicity caused by TAA.
本研究旨在评估芦丁对硫代乙酰胺（TAA）诱导的大鼠肝毒性的影响。使用了四组雄性 Wistar 大鼠，每组六只：组 I：对照组；组 II：大鼠单次腹腔注射 300mg/kg 体重的 TAA；组 III：大鼠口服芦丁（10mg/kg 体重）溶于生理盐水 2 周；组 IV：大鼠口服芦丁（10mg/kg 体重）溶于生理盐水 2 周，然后在第 2 周的最后一天注射 TAA。所有组在治疗后 24 小时内处死，并分析肝毒性标志物、肝 DNA 片段化和肝组织学。在 Wistar 大鼠中给予 TAA 导致肝标志物显著增加、肝细胞 DNA 片段化和组织学改变。在 TAA 攻击前 2 周用芦丁预处理大鼠可完全逆转 TAA 介导的肝毒性（P<0.0001 至 P<0.01），同时恢复 DNA 片段化。本研究表明芦丁是一种保护剂，可恢复 TAA 引起的毒性。
