Department of Biochemistry and Orthodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan, JP.
Lasers Med Sci. 2000 Jan;15(1):35-42. doi: 10.1007/s101030050045.
Human gingival fibroblasts (hGF) reside in gingival tissues which are challenged frequently by oral bacteria. Lipopolysaccharide (LPS) from periodontal pathogens can penetrate gingival tissues and stimulate plasminogen activator (PA) activity which is implicated in the PA/plasmin proteolytic system. The PA/plasmin system plays an important role in the degradation of the extracellular matrix and synthesis of kinin in the process of inflammation. Anti-inflammatory effects by low-energy laser irradiation have been reported. However, the mechanisms of biostimulatory effects have not been elucidated in detail. The primarily cultured hGF cells were challenged with LPS isolated from Campylobacter rectus which was known as a periodontal pathogen and Ga-Al-As diode low energy laser was irradiated (830 nm, 7.90 J/cm(2)). The cultured medium of hGF cells showed a marked elevation in PA activity by LPS, which was significantly inhibited by the laser irradiation in a dose-dependent manner. This effect was involved in the reduction of tPA mRNA levels.
人牙龈成纤维细胞（hGF）位于牙龈组织中，这些组织经常受到口腔细菌的挑战。牙周病病原体的脂多糖（LPS）可以穿透牙龈组织并刺激纤溶酶原激活物（PA）的活性，该活性与 PA/纤溶酶蛋白水解系统有关。PA/纤溶酶系统在炎症过程中细胞外基质的降解和激肽的合成中发挥重要作用。已经报道了低能量激光照射的抗炎作用。然而，其生物刺激作用的机制尚未详细阐明。主要培养的 hGF 细胞用已知的牙周病病原体弯曲杆菌属 LPS 刺激，并用 Ga-Al-As 二极管低能量激光照射（830nm，7.90J/cm(2)）。hGF 细胞的培养介质中 LPS 引起的 PA 活性明显升高，激光照射以剂量依赖性方式显著抑制该活性。这种作用涉及 tPA mRNA 水平的降低。
