Kuboyama Noboru, Ohta Mitsuhiro, Sato Yusuke, Abiko Yoshimitsu
Department of Pharmacology, Nihon University School of Dentistry at Matsudo ; Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo.
Department of Oral Diagnosis, Nihon University School of Dentistry at Matsudo ; Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo.
Laser Ther. 2014 Sep 30;23(3):191-9. doi: 10.5978/islsm.14-OR-15.
Rheumatoid arthritis (RA) is an auto-immune disease afflicting multiple joints of the body, where as a result of the increase in inflammatory cytokines and tissue destructive factors such as matrix metalloproteinase (MMP)-3, deterioration of the bones and cartilages of the joints occurs. The present investigation was carried out to study the anti-inflammatory activities of light emitting diode (LED) irradiation on hind paw inflammation in collagen-induced arthritis (CIA) mice models.
RA in the CIA mouse model was induced by immunization of DBA/1J mice with intradermal injections of an emulsion of bovine type II collagen and complete Freund's adjuvant. A total of 20 CIA mice were subdivided into the following groups: control group, CIA group and 2 groups of LED irradiated CIA mice (LED groups) (n=5 per group). The mouse knee joint area in the LED groups (the 570 nm and 940 nm groups) was irradiated with LED energy, three times a week for 500 s per session over 8 weeks at a dose of 5 J/cm(2). The hind paw swelling was assessed by the increase in hind paw thickness. The serum levels of the inflammatory cytokines and arthritic factor MMP-3 were determined with an enzyme-linked immunosorbent assay (ELISA).
In the LED-570 and LED-940 groups at 4 weeks after arthritis induction, the swelling inhibition index was 18.1±4.9 and 29.3±4.0 respectively. Interleukin (IL)-1β, IL-6 and MMP-3 serum levels were significantly lower in the LED-940 group.
LED irradiation, particularly in the near-infrared was effective for inhibition of the inflammatory reactions caused by RA.
类风湿性关节炎（RA）是一种影响身体多个关节的自身免疫性疾病，由于炎症细胞因子和组织破坏因子如基质金属蛋白酶（MMP）-3的增加，关节的骨骼和软骨会发生退化。本研究旨在探讨发光二极管（LED）照射对胶原诱导性关节炎（CIA）小鼠模型后爪炎症的抗炎活性。
通过对DBA/1J小鼠皮内注射牛II型胶原和完全弗氏佐剂乳液诱导CIA小鼠模型中的RA。总共20只CIA小鼠被分为以下几组：对照组、CIA组和2组LED照射的CIA小鼠（LED组）（每组n = 5）。LED组（570 nm和940 nm组）的小鼠膝关节区域用LED能量照射，每周3次，每次500秒，持续8周，剂量为5 J/cm²。通过后爪厚度的增加评估后爪肿胀情况。用酶联免疫吸附测定（ELISA）测定炎症细胞因子和关节炎因子MMP-3的血清水平。
在关节炎诱导后4周，LED-570组和LED-940组的肿胀抑制指数分别为18.1±4.9和29.3±4.0。LED-940组的白细胞介素（IL）-1β、IL-6和MMP-3血清水平显著降低。
LED照射，尤其是近红外照射，对抑制RA引起的炎症反应有效。
