Kitano K, Suda T, Sato Y, Miura Y
Department of Medicine, Jichi Medical School, Tochigi-ken.
Jpn J Cancer Res. 1988 Jul;79(7):843-9. doi: 10.1111/j.1349-7006.1988.tb00046.x.
The effect of human granulocyte colony-stimulating factor (G-CSF) on leukemic cells of acute promyelocytic leukemia (APL) was examined. Mononuclear cells obtained from bone marrow cells containing more than 90% blasts from seven APL patients were incubated in the presence of G-CSF using semisolid and liquid culture systems. On day 7, the cells from all the patients produced many clusters consisting of 8-40 cells. These cells appeared to be promyelocyte-like blast cells in four patients and had differentiated to more mature neutrophils in three patients. On day 14, the number of clusters decreased except for two patients. Blast cells from the two patients showing the increase of blast clusters could proliferate in a liquid culture containing G-CSF. Blast cells cultured for 14 days formed many secondary cultures after replating on a methylcellulose medium. Moreover, chromosomal analyses of blasts cultivated in the presence of G-CSF for 7 days showed t(15;17) in all metaphases in one patient. It appears that the leukemic cells from APL patients could proliferate in the presence of G-CSF.
研究了人粒细胞集落刺激因子（G-CSF）对急性早幼粒细胞白血病（APL）白血病细胞的作用。从7例APL患者的骨髓细胞中获取单核细胞，这些骨髓细胞中原始细胞含量超过90%，使用半固体和液体培养系统在G-CSF存在的情况下进行培养。第7天，所有患者的细胞均产生了许多由8 - 40个细胞组成的集落。在4例患者中，这些细胞似乎是早幼粒细胞样原始细胞，而在3例患者中已分化为更成熟的中性粒细胞。第14天，除2例患者外，集落数量减少。显示原始细胞集落增加的2例患者的原始细胞可在含有G-CSF的液体培养中增殖。在甲基纤维素培养基上重新接种后，培养14天的原始细胞形成了许多传代培养物。此外，对在G-CSF存在下培养7天的原始细胞进行染色体分析，发现1例患者的所有中期分裂相中均存在t(15;17)。看来APL患者的白血病细胞在G-CSF存在的情况下能够增殖。
