Nakamaki T, Sakashita A, Sano M, Hino K, Suzuki K, Tomoyasu S, Tsuruoka N, Honma Y, Hozumi M
Department of Hematology, Showa University School of Medicine, Tokyo.
Jpn J Cancer Res. 1989 Nov;80(11):1077-82. doi: 10.1111/j.1349-7006.1989.tb02262.x.
The interaction of granulocyte-colony stimulating factor (G-CSF) and retinoic acid (RA) in proliferation and differentiation of acute promyelocytic leukemia (APL) cells was examined. G-CSF stimulated proliferation of APL cells at concentrations of 0.1 to 50 ng/ml in a dose dependent manner. More than 10(-8) M RA induced granulocytic differentiation of APL cells. Although G-CSF induced lysozyme activities in APL cells, it alone did not induce terminal differentiation of APL cells. G-CSF significantly enhanced the RA-induced granulocytic differentiation of APL cells in vitro. Enhancement by G-CSF was not due to the prolongation of survival of RA-induced differentiated cells, but the differentiation-inducing effects of G-CSF might be evident only in the presence of RA. Since G-CSF has a potential to induce the granulocytic differentiation of myeloid leukemia cells, G-CSF in combination with RA may be applicable in differentiation induction therapy for some types of myeloid leukemia.
研究了粒细胞集落刺激因子（G-CSF）与视黄酸（RA）在急性早幼粒细胞白血病（APL）细胞增殖和分化中的相互作用。G-CSF在0.1至50 ng/ml浓度下以剂量依赖性方式刺激APL细胞增殖。超过10^(-8) M的RA诱导APL细胞向粒细胞分化。虽然G-CSF诱导APL细胞中的溶菌酶活性，但它单独不能诱导APL细胞的终末分化。G-CSF在体外显著增强了RA诱导的APL细胞向粒细胞分化。G-CSF的增强作用不是由于RA诱导的分化细胞存活时间延长，而是G-CSF的分化诱导作用可能仅在RA存在时才明显。由于G-CSF有诱导髓系白血病细胞向粒细胞分化的潜力，G-CSF与RA联合可能适用于某些类型髓系白血病的分化诱导治疗。
