Botsali Murat Selim, Kuşgöz Adem, Altintaş Subutay Han, Ülker Hayriye Esra, Tanriver Mehmet, Kiliç Serdar, Başak Feridun, Ülker Mustafa
Department of Pedodontics, Faculty of Dentistry, Selcuk University, Selcuklu 42079, Konya, Turkey.
Department of Pedodontics, Faculty of Dentistry, Karadeniz Technical University, 61080 Trabzon, Turkey.
ScientificWorldJournal. 2014 Jan 28;2014:218295. doi: 10.1155/2014/218295. eCollection 2014.
The purpose of this study was first to evaluate the elution of 2-hydroxyethyl methacrylate (HEMA) and triethylene glycol dimethacrylate (TEGDMA) monomers from resin-modified glass ionomer cement (RMGIC) and compomers cured with halogen and light-emitting diode (LED) light-curing units (LCUs). The effect of cured materials on the viability of L929 fibroblast cells was also evaluated. One RMGIC (Ketac N100) and two compomers (Dyract Extra and Twinkystar) were tested. Materials were prepared in teflon disks and light-cured with LED or halogen LCUs. The residual monomers of resin materials in solution were identified using high-performance liquid chromatography. The fibroblast cells' viability was analyzed using MTT assay. The type of LCU did not have a significant effect on the elution of HEMA and TEGDMA. A greater amount of HEMA than TEGMDA was eluted. The amount of TEGDMA eluted from Twinkystar was greater than Dyract Extra (P < 0.05) when cured with a halogen LCU. All material-LCU combinations decreased the fibroblast cells' viability more than the control group (P < 0.01), except for Dyract Extra cured with a halogen LCU (P > 0.05). Curing with the LED LCU decreased the cells' viability more than curing with the halogen LCU for compomers. For Ketac N100, the halogen LCU decreased the cells' viability more than the LED LCU.
本研究的目的首先是评估用卤素和发光二极管（LED）光固化灯（LCU）固化的树脂改性玻璃离子水门汀（RMGIC）和复合体中甲基丙烯酸羟乙酯（HEMA）和二缩三乙二醇二甲基丙烯酸酯（TEGDMA）单体的洗脱情况。还评估了固化材料对L929成纤维细胞活力的影响。测试了一种RMGIC（Ketac N100）和两种复合体（Dyract Extra和Twinkystar）。材料制备在聚四氟乙烯盘中，并用LED或卤素LCU进行光固化。使用高效液相色谱法鉴定溶液中树脂材料的残留单体。使用MTT法分析成纤维细胞的活力。LCU的类型对HEMA和TEGDMA的洗脱没有显著影响。洗脱的HEMA量比TEGDMA多。当用卤素LCU固化时，从Twinkystar洗脱的TEGDMA量大于Dyract Extra（P < 0.05）。除了用卤素LCU固化的Dyract Extra（P > 0.05）外，所有材料-LCU组合均比对照组更能降低成纤维细胞的活力（P < 0.01）。对于复合体，用LED LCU固化比用卤素LCU固化更能降低细胞活力。对于Ketac N100，卤素LCU比LED LCU更能降低细胞活力。
