YgaE在高渗胁迫下调节伤寒沙门氏菌的外膜蛋白。
YgaE regulates out membrane proteins in Salmonella enterica serovar Typhi under hyperosmotic stress.
作者信息
Wang Min, Feng Ping, Chen Xun, Zhang Haifang, Ni Bin, Xie Xiaofang, Du Hong
机构信息
Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
Clinical Laboratory Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
出版信息
ScientificWorldJournal. 2014 Jan 23;2014:374276. doi: 10.1155/2014/374276. eCollection 2014.
Salmonella enterica serovar Typhi (S. Typhi) is a human-specific pathogen that causes typhoid fever. In this study, we constructed ΔygaE mutant and a microarray was performed to investigate the role of ygaE in regulation of gene expression changes in response to hyperosmotic stress in S. Typhi. qRT-PCR was performed to validate the microarray results. Our data indicated that ygaE was the repressor of gab operon in S. Typhi as in Escherichia coli (E. coli), though the sequence of ygaE is totally different from gabC (formerly ygaE) in E. coli. OmpF, OmpC, and OmpA are the most abundant out membrane proteins in S. Typhi. Here we report that YgaE is a repressor of both OmpF and OmpC at the early stage of hyperosmotic stress. Two-dimensional electrophoresis was applied to analyze proteomics of total proteins in wild-type strain and ΔygaE strain and we found that YgaE represses the expression of OmpA at the late stage of hyperosmotic stress. Altogether, our results implied that YgaE regulates out membrane proteins in a time-dependent manner under hyperosmotic stress in S. Typhi.
伤寒沙门氏菌血清型伤寒杆菌（伤寒杆菌）是一种引起伤寒热的人类特异性病原体。在本研究中，我们构建了ΔygaE突变体，并进行了微阵列分析，以研究ygaE在伤寒杆菌中对高渗胁迫响应的基因表达变化调控中的作用。进行了qRT-PCR以验证微阵列结果。我们的数据表明，尽管ygaE的序列与大肠杆菌中的gabC（以前的ygaE）完全不同，但ygaE在伤寒杆菌中与在大肠杆菌中一样，是gab操纵子的阻遏物。OmpF、OmpC和OmpA是伤寒杆菌中最丰富的外膜蛋白。在此我们报告，在高渗胁迫早期，YgaE是OmpF和OmpC两者的阻遏物。应用二维电泳分析野生型菌株和ΔygaE菌株中总蛋白的蛋白质组学，我们发现YgaE在高渗胁迫后期抑制OmpA的表达。总之，我们的结果表明，在伤寒杆菌的高渗胁迫下，YgaE以时间依赖性方式调节外膜蛋白。
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