Kaufmann S H, Hahn H, Berger R, Kirchner H
Eur J Immunol. 1983 Mar;13(3):265-8. doi: 10.1002/eji.1830130318.
Cultures of peritoneal exudate T lymphocyte-enriched cells (PETLEC) from Listeria monocytogenes-immune mice, antigen-presenting cells (APC) and heat-killed L. monocytogenes produced high amounts of interferon-gamma (IFN-gamma). High IFN titers were also observed after stimulation of L. monocytogenes-immune cell cultures with the T cell mitogens concanavalin A and phytohemagglutinin. L. monocytogenes-immune PETLEC produced several fold higher IFN titers than normal cell cultures in response to mitogen and antigen. Under both circumstances, APC were required for optimum responses. L. monocytogenes-immune PETLEC participating in IFN production were Lyt 1+23-. IFN-gamma was also produced in cultures of cloned L. monocytogenes-specific T cells. Since the same T cell clone showed antigen-specific proliferative responses and interleukin production in vitro, and could adoptively mediate delayed-type hypersensitivity and anti-listerial protection in vivo, it is suggested that IFN production is a function of specific T cells active in cellular antibacterial immunity.
来自单核细胞增生李斯特菌免疫小鼠的腹膜渗出液富含T淋巴细胞的细胞（PETLEC）、抗原呈递细胞（APC）与热灭活的单核细胞增生李斯特菌共培养时会产生大量的γ干扰素（IFN-γ）。在用T细胞有丝分裂原刀豆蛋白A和植物血凝素刺激单核细胞增生李斯特菌免疫细胞培养物后，也观察到了高IFN滴度。单核细胞增生李斯特菌免疫的PETLEC在对有丝分裂原和抗原的反应中产生的IFN滴度比正常细胞培养物高几倍。在这两种情况下，都需要APC才能获得最佳反应。参与IFN产生的单核细胞增生李斯特菌免疫的PETLEC是Lyt 1+23-。克隆的单核细胞增生李斯特菌特异性T细胞培养物中也产生IFN-γ。由于同一个T细胞克隆在体外表现出抗原特异性增殖反应和白细胞介素产生，并且在体内可以过继介导迟发型超敏反应和抗李斯特菌保护作用，因此提示IFN产生是参与细胞抗菌免疫的特异性T细胞的功能。
