Yan Yan-Hong, Li Jun-Lin, Zhang Xin-Quan, Yang Wen-Yu, Wan Yan, Ma Ying-Mei, Zhu Yong-Qun, Peng Yan, Huang Lin-Kai
Department of Grassland Science, College of Animal Science and Technology, Sichuan Agriculture University, Ya'an, Sichuan, China.
College of Agronomy, Sichuan Agricultural University, Wenjiang, Sichuan, China.
PLoS One. 2014 Mar 4;9(3):e90700. doi: 10.1371/journal.pone.0090700. eCollection 2014.
In order to find a way to induce rooting on cuttings of Hemarthria compressa cv. Ya'an under controlled conditions, a project was carried out to study the effect of naphthalene acetic acid (NAA) on rooting in stem cuttings and related physiological changes during the rooting process of Hemarthria compressa. The cuttings were treated with five concentrations of NAA (0, 100, 200 300, 400 mg/l) at three soaking durations (10, 20, 30 minutes), and cuttings without treatment were considered as control. Samples were planted immediately into pots after treatment. IAA-oxidase (IAAO) activity, peroxidase (POD) activity and polyphenol oxidase (PPO) activity were determined after planting. Results showed that NAA had positive effect on rooting at the concentration of 200 mg/l compared to other concentrations at 30 days after planting (DAP). Among the three soaking durations, 20 minutes (min) of 200 mg/l NAA resulted in higher percentages of rooting, larger numbers of adventitious roots and heavier root dry weight per cutting. The lowest IAAO activity was obtained when soaked at 200 mg/l NAA for 20 min soaking duration. This was consistent with the best rooting ability, indicating that the lower IAAO activity, the higher POD activity and PPO activity could be used as an indicator of better rooting ability for whip grass cuttings and might serve as a good marker for rooting ability in cuttings.
为了找到在可控条件下诱导雅安扁穗牛鞭草插条生根的方法，开展了一项研究萘乙酸（NAA）对扁穗牛鞭草茎插条生根及生根过程中相关生理变化影响的项目。插条用五种浓度的NAA（0、100、200、300、400毫克/升）在三个浸泡时长（10、20、30分钟）下进行处理，未处理的插条作为对照。处理后立即将插条种植到花盆中。种植后测定吲哚乙酸氧化酶（IAAO）活性、过氧化物酶（POD）活性和多酚氧化酶（PPO）活性。结果表明，与种植后30天的其他浓度相比，200毫克/升浓度的NAA对生根有积极影响。在三个浸泡时长中，200毫克/升NAA浸泡20分钟导致生根率更高、不定根数量更多且每插条根干重更重。当在200毫克/升NAA中浸泡20分钟时，IAAO活性最低。这与最佳生根能力一致，表明较低的IAAO活性、较高的POD活性和PPO活性可作为扁穗牛鞭草插条生根能力较好的指标，并且可能是插条生根能力的良好标志物。
