Dinçalp Haluk, Kızılok Şevki, İçli Sıddık
Department of Chemistry, Faculty of Arts and Science, Celal Bayar University, Muradiye, 45030, Manisa, Turkey,
J Fluoresc. 2014 May;24(3):917-24. doi: 10.1007/s10895-014-1372-5. Epub 2014 Mar 5.
Singlet oxygen quantum yields (ΦΔ) of different perylene diimides (PDIs) containing phenyl (PDI-Ph), pyrene (PDI-Pyr), and indole (PDI-In) units in bay positions of the ring were determined using 1,3-diphenylisobenzofuran (DPBF) method in toluene/methanol (99:1) system. Pyrene-substituted PDI were the most efficient singlet oxygen generator among the investigated photosensitizers with a quantum yield of Φ Δ = 0.93 in toluene/methanol. Additionally, their binding affinities to G-quadruplex DNA structure were investigated by steady-state measurements. There were marked red shifts of absorbance bands for PDI-Pyr/DNA strand complexes with respect to the absorption maxima of DNA-free solution of PDI-Pyr in phosphate buffer at pH 6.
使用1,3 - 二苯基异苯并呋喃（DPBF）法，在甲苯/甲醇（99:1）体系中测定了环的湾区含有苯基（PDI-Ph）、芘（PDI-Pyr）和吲哚（PDI-In）单元的不同苝二酰亚胺（PDIs）的单线态氧量子产率（ΦΔ）。在所研究的光敏剂中，芘取代的PDI是最有效的单线态氧产生剂，在甲苯/甲醇中的量子产率为ΦΔ = 0.93。此外，通过稳态测量研究了它们与G-四链体DNA结构的结合亲和力。相对于pH 6的磷酸盐缓冲液中不含DNA的PDI-Pyr溶液的吸收最大值，PDI-Pyr/DNA链复合物的吸收带出现了明显的红移。
