Department of Biology, Keimyung University, Daegu 704-701, South Korea.
Saudi J Biol Sci. 2014 Jan;21(1):89-97. doi: 10.1016/j.sjbs.2013.06.002. Epub 2013 Jun 15.
The effect of glutathione on the influences of heavy metals affecting rubisco and rubisco activase was studied in tobacco plants grown in vitro where the shoot explants of the tobacco plant cultured on MS medium under aseptic conditions and two explants were placed in the control, 0.1 mM GSH, 1 mM GSH, 0.2 mM Cd, 0.2 mM Cu, 0.2 mM Zn, and a mixture of Cd and GSH, Cu and GSH, Zn and GSH, respectively. The effect of GSH on the growth of the tobacco plant was minimal, but the heavy metals clearly retarded its growth. GSH recovered the growth retarded by heavy metals, and the concentration of GSH required to recover the growth differed depending on the heavy metals. The content of chlorophyll in the plant increased through GSH and Zn, and decreased through Cd and Cu. The chlorophyll content which decreased due to Cd and Cu was recovered by GSH, and the content which increased due to Zn was decreased by 1 mM GSH. The content of rubisco decreased due to GSH and heavy metals, and the content which decreased due to heavy metals was recovered by GSH, and when GSH was treated with Zn, the increased rate was maximum compared to other heavy metals. The activity of rubisco was increased due to GSH and heavy metals, and the activity increased by Cd and Zn decreased through GSH. In the case of Cu, the activity of GSH increased even more. There was no effect of GSH on the influences of heavy metals on the content and activity of rubisco activase. The activity of rubisco decreased by thiourea among six denaturing agents, and increased by l-cysteine, and in most cases the activity level was recorded as high. The activity of rubisco activase all decreased as a result of six denaturing agents, and the effect caused by EDTA and guanidine-HCl was the greatest, while the effect caused by l-cysteine and urea was minimal.
研究了在离体条件下生长的烟草植物中，谷胱甘肽对重金属影响 Rubisco 和 Rubisco 激活酶的影响。在无菌条件下，将烟草植物的茎外植体培养在 MS 培养基上，培养两个外植体，分别置于对照、0.1 mM GSH、1 mM GSH、0.2 mM Cd、0.2 mM Cu、0.2 mM Zn 和 Cd 和 GSH、Cu 和 GSH、Zn 和 GSH 的混合物中。GSH 对烟草植物生长的影响最小，但重金属明显抑制其生长。GSH 恢复了重金属抑制的生长，恢复生长所需的 GSH 浓度取决于重金属。植物中的叶绿素含量通过 GSH 和 Zn 增加，通过 Cd 和 Cu 减少。由于 Cd 和 Cu 减少的叶绿素含量通过 GSH 恢复，由于 Zn 增加的含量通过 1 mM GSH 减少。Rubisco 的含量由于 GSH 和重金属而减少，由于重金属而减少的含量通过 GSH 恢复，当 GSH 与 Zn 一起处理时，与其他重金属相比，增加率最大。Rubisco 的活性由于 GSH 和重金属而增加，由于 Cd 和 Zn 增加的活性通过 GSH 降低。在 Cu 的情况下，GSH 的活性甚至增加。GSH 对重金属对 Rubisco 激活酶含量和活性的影响没有影响。六种变性剂中，硫脲使 Rubisco 的活性降低，l-半胱氨酸使其增加，大多数情况下，活性水平记录为高。六种变性剂均使 Rubisco 激活酶的活性降低，EDTA 和盐酸胍的作用最大，而 l-半胱氨酸和尿素的作用最小。
