Fang Xiangling, Zhang Manrang, Tang Qian, Wang Yonghong, Zhang Xing
1] Research and Development Center of Biorational Pesticides, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi 712100, China [2].
1] College of Horticulture, Northwest A&F University, 3 Taicheng Road, Yangling, Shaanxi 712100, China [2].
Sci Rep. 2014 Mar 6;4:4300. doi: 10.1038/srep04300.
Entomopathogenic bacteria Xenorhabdus spp. produce secondary metabolites with potential antimicrobial activity for use in agricultural productions. This study evaluated the inhibitory effect of X. nematophila TB culture on plant pathogens Botrytis cinerea and Phytophthora capsici. The cell-free filtrate of TB culture showed strong inhibitory effects (>90%) on mycelial growth of both pathogens. The methanol-extracted bioactive compounds (methanol extract) of TB culture also had strong inhibitory effects on mycelial growth and spore germinations of both pathogens. The methanol extract (1000 μg/mL) and cell-free filtrate both showed strong therapeutic and protective effects (>70%) on grey mold both in detached tomato fruits and plants, and leaf scorch in pepper plants. This study demonstrates X. nematophila TB produces antimicrobial metabolites of strong activity on plant pathogens, with great potential for controlling tomato grey mold and pepper leaf scorch and being used in integrated disease control to reduce chemical application.
昆虫病原细菌嗜线虫致病杆菌属（Xenorhabdus spp.）产生具有潜在抗菌活性的次生代谢产物，可用于农业生产。本研究评估了嗜线虫致病杆菌TB菌株培养物对植物病原菌灰葡萄孢菌（Botrytis cinerea）和辣椒疫霉（Phytophthora capsici）的抑制作用。TB菌株培养物的无细胞滤液对两种病原菌的菌丝生长均表现出强烈的抑制作用（>90%）。TB菌株培养物的甲醇提取物（甲醇萃取物）对两种病原菌的菌丝生长和孢子萌发也有强烈的抑制作用。甲醇提取物（1000 μg/mL）和无细胞滤液对离体番茄果实和植株上的灰霉病以及辣椒植株上的叶斑病均表现出强烈的治疗和保护作用（>70%）。本研究表明，嗜线虫致病杆菌TB菌株产生对植物病原菌具有强活性的抗菌代谢产物，在防治番茄灰霉病和辣椒叶斑病以及用于综合病害防治以减少化学药剂施用方面具有巨大潜力。
