Paixão Paulo, Piedade Cátia, Papoila Ana, Caires Iolanda, Pedro Catarina, Santos Madalena, Silvestre Maria José, Brum Laura, Nunes Baltazar, Guiomar Raquel, Curran Martin D, Carvalho Ana, Marques Teresa, Neuparth Nuno
CEDOC, Faculdade de Ciências Médicas, FCM, Universidade Nova de Lisboa, 1169-056, Lisboa, Portugal,
Eur J Pediatr. 2014 Aug;173(8):1059-65. doi: 10.1007/s00431-014-2285-7. Epub 2014 Mar 6.
Influenza surveillance is usually based on nationally organized sentinel networks of physicians and on hospital reports. This study aimed to test a different report system, based on parents' phone contact to the research team and in home collection of samples by a dedicated team. The identification of influenza and other respiratory viruses in children who attended a Hospital Emergency Department was also recorded. Real-time PCR and reverse transcription PCR were performed for influenza A and B, parainfluenza 1-4, adenovirus, human metapneumovirus, respiratory syncytial virus A and B, rhinovirus, enterovirus, group 1 coronaviruses, group 2 coronaviruses, and human bocavirus. One hundred children were included, 64 from the day care centers and 36 from the Hospital. Overall, 79 samples were positive for at least one respiratory virus. Influenza A (H3) was the virus most frequently detected: 25 cases, 20 of these in children under 5 years of age (ten from day care centers and ten who went to the hospital) which was higher than those reported by the National Influenza Surveillance Programme for this age.
The results obtained in this study suggest that a surveillance system based on parents' reports could complement the implanted system of the National Influenza Surveillance Programme.
流感监测通常基于全国组织的医生哨点网络和医院报告。本研究旨在测试一种不同的报告系统,该系统基于家长与研究团队的电话联系以及由专门团队进行的家庭样本采集。还记录了在医院急诊科就诊的儿童中流感和其他呼吸道病毒的鉴定情况。对甲型和乙型流感、副流感1 - 4型、腺病毒、人偏肺病毒、呼吸道合胞病毒A和B、鼻病毒、肠道病毒、1组冠状病毒、2组冠状病毒和人博卡病毒进行了实时PCR和逆转录PCR检测。纳入了100名儿童,其中64名来自日托中心,36名来自医院。总体而言,79份样本至少对一种呼吸道病毒呈阳性。甲型流感(H3)是最常检测到的病毒:25例,其中20例为5岁以下儿童(10例来自日托中心,10例前往医院),这高于国家流感监测计划针对该年龄段报告的病例数。
本研究获得的结果表明,基于家长报告的监测系统可以补充国家流感监测计划已实施的系统。