Department of Experimental Modeling and Pathogenesis of Infectious Diseases, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.
Department of Propaedeutic of Childhood Diseases, Novosibirsk State Medical University, Novosibirsk, Russia.
PLoS One. 2018 Sep 18;13(9):e0200117. doi: 10.1371/journal.pone.0200117. eCollection 2018.
Acute respiratory infections (ARIs) cause a considerable morbidity and mortality worldwide especially in children. However, there are few studies of the etiological structure of ARIs in Russia. In this work, we analyzed the etiology of ARIs in children (0-15 years old) admitted to Novosibirsk Children's Municipal Clinical Hospital in 2013-2017.
We tested nasal and throat swabs of 1560 children with upper or lower respiratory infection for main respiratory viruses (influenza viruses A and B, parainfluenza virus types 1-4, respiratory syncytial virus, metapneumovirus, four human coronaviruses, rhinovirus, adenovirus and bocavirus) using a RT-PCR Kit.
We detected 1128 (72.3%) samples were positive for at least one virus. The most frequently detected pathogens were respiratory syncytial virus (358/1560, 23.0%), influenza virus (344/1560, 22.1%), and rhinovirus (235/1560, 15.1%). Viral co-infections were found in 163 out of the 1128 (14.5%) positive samples. We detected significant decrease of the respiratory syncytial virus-infection incidence in children with increasing age, while the reverse relationship was observed for influenza viruses.
We evaluated the distribution of respiratory viruses in children with ARIs and showed the prevalence of respiratory syncytial virus and influenza virus in the etiological structure of infections. This study is important for the improvement and optimization of diagnostic tactics, control and prevention of the respiratory viral infections.
急性呼吸道感染（ARI）在全球范围内导致了相当高的发病率和死亡率，尤其是在儿童中。然而，俄罗斯关于 ARI 病因结构的研究很少。在这项工作中，我们分析了 2013 年至 2017 年期间在新西伯利亚市立儿童医院就诊的儿童（0-15 岁）ARI 的病因。
我们使用 RT-PCR 试剂盒检测了 1560 例上呼吸道或下呼吸道感染儿童的鼻拭子和咽拭子，以检测主要呼吸道病毒（甲型和乙型流感病毒、副流感病毒 1-4 型、呼吸道合胞病毒、副黏液病毒、四种人类冠状病毒、鼻病毒、腺病毒和博卡病毒）。
我们检测到 1128 份（72.3%）样本至少有一种病毒呈阳性。最常检测到的病原体是呼吸道合胞病毒（358/1560，23.0%）、流感病毒（344/1560，22.1%）和鼻病毒（235/1560，15.1%）。在 1128 份阳性样本中，有 163 份（14.5%）检测到病毒合并感染。我们发现，随着儿童年龄的增长，呼吸道合胞病毒感染的发病率显著下降，而流感病毒则呈相反的关系。
我们评估了 ARI 儿童中呼吸道病毒的分布，并显示了呼吸道合胞病毒和流感病毒在感染病因结构中的流行。这项研究对于改进和优化诊断策略、控制和预防呼吸道病毒感染具有重要意义。
