Department of Chemical Engineering, Pohang University of Science and Technology , Pohang 790-784, Korea.
Biomacromolecules. 2014 Apr 14;15(4):1390-8. doi: 10.1021/bm500001n. Epub 2014 Mar 18.
Silk has recently been exploited in various fields due to its superior mechanical properties. However, this material's lack of biological functions and relatively poor biodegradation have hindered its wide use in applications related to cells and tissues. Here, we improved the overall characteristics of silkworm silk fibroin (SF) by introduction of RGD peptide-fused recombinant mussel adhesive protein (MAP-RGD). Simple blending of MAP-RGD provided not only bulk-scale adhesive ability but also microscale adhesiveness to cells and various biomolecules. MAP-RGD-blended SF fibers supported enhanced adhesion, proliferation, and spreading of mammalian cells as well as the efficient attachment of biomolecules, including carbohydrate and protein. In addition, the hydrophilicity, swelling, and biodegradability of the MAP-RGD-blended SF material were improved without notable hampering of the original mechanical properties of SF. Therefore, the adhesive silk fibrous scaffold could be successfully used in diverse biomedical engineering applications.
由于其优越的机械性能，丝绸最近已在各个领域得到了广泛的应用。然而，这种材料缺乏生物功能，生物降解性相对较差，限制了其在与细胞和组织相关的应用中的广泛使用。在这里，我们通过引入 RGD 肽融合重组贻贝类黏附蛋白（MAP-RGD）来改善家蚕丝素纤维（SF）的整体特性。MAP-RGD 的简单共混不仅提供了大规模的黏附能力，还提供了对细胞和各种生物分子的微观黏附性。MAP-RGD 共混 SF 纤维支持哺乳动物细胞的增强黏附、增殖和扩散，以及生物分子（包括碳水化合物和蛋白质）的有效附着。此外，MAP-RGD 共混 SF 材料的亲水性、溶胀性和生物降解性得到了改善，而 SF 材料的原始机械性能没有明显受到影响。因此，黏附性丝纤维支架可成功用于各种生物医学工程应用。
