右移葡聚糖血红蛋白作为血液替代品。

Rightshifted dextran-hemoglobin as blood substitute.

作者信息

Wong J T

机构信息

Department of Biochemistry, University of Toronto, Canada.

出版信息

Biomater Artif Cells Artif Organs. 1988;16(1-3):237-45. doi: 10.3109/10731198809132573.

DOI:10.3109/10731198809132573

PMID:2460165

Abstract

Covalent modification of hemoglobin and dextran-hemoglobin by reductive alkylation under aerobic conditions with periodated inositol tetrakisphosphate brought about a rightshifting of their oxygen dissociation curves. The rightshifted dextran-hemoglobin conjugate provided adequate oxygen delivery in macaques exchange-transfused to 90% with respect to their erythrocyte content. The conjugate did not exhibit the nephrotoxicity of free hemoglobin. It was also more resistant to precipitation, and therefore more amenable to viral sterilisation, by ethanol.

摘要

在有氧条件下，用高碘酸肌醇四磷酸通过还原烷基化对血红蛋白和葡聚糖-血红蛋白进行共价修饰，导致它们的氧解离曲线右移。右移的葡聚糖-血红蛋白共轭物在猕猴中红细胞含量被置换90%的交换输血中提供了充足的氧输送。该共轭物未表现出游离血红蛋白的肾毒性。它对沉淀也更具抗性，因此更适合通过乙醇进行病毒灭活。