可溶性葡聚糖-血红蛋白复合物作为一种潜在的血液替代品。
Soluble dextran-hemoglobin complex as a potential blood substitute.
作者信息
Tam S C, Blumenstein J, Wong J T
出版信息
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2128-31. doi: 10.1073/pnas.73.6.2128.
Abstract
A complex between soluble dextran and human hemoglobin has been synthesized by two different methods. In the alkylation method, hemoglobin was allowed to react with bromoacetyl groups incorporated into the dextran; the yield of the complex was about 80% in terms of the hemoglobin used. In the dialdehyde method, hemoglobin was allowed to react with dialdehyde groups on the dextran generated by periodate oxidation; the yield of the complex was about 60%. Both soluble dextran-hemoglobin complexes could bind and release oxygen reversibly, but the oxygen-binding curves were shifted to the left relative to that of free hemoglobin. In the rabbit, the complex obtained by the alkylation method was excreted by the kidneys and cleared from the circulation much more slowly than free hemoglobin.
摘要
通过两种不同方法合成了可溶性葡聚糖与人类血红蛋白的复合物。在烷基化方法中，使血红蛋白与引入葡聚糖中的溴乙酰基反应；以所用血红蛋白计，复合物的产率约为80%。在二醛法中，使血红蛋白与由高碘酸盐氧化产生的葡聚糖上的二醛基反应；复合物的产率约为60%。两种可溶性葡聚糖-血红蛋白复合物均可可逆地结合和释放氧气，但与游离血红蛋白相比，氧结合曲线向左移动。在兔体内，通过烷基化方法获得的复合物由肾脏排泄，从循环中清除的速度比游离血红蛋白慢得多。
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