Tam S C, Blumenstein J, Wong J T
Can J Biochem. 1978 Oct;56(10):981-4. doi: 10.1139/o78-153.
Exchange transfusions in dogs were performed with a solution of either dextran or a covalent complex between dextran and human hemoglobin. Dogs transfused with dextran alone died when their hematocrit was lowered to 6-10%. Dogs transfused with dextran-hemoglobin complex, however, survived a reduction of their hematocrit to 2% or below. In the latter animals, the dextran-hemoglobin complex disappeared from the circulation with an average half-life of 2.4 days. Correcting for oxidation of the hemoglobin moiety to methemoglobin, the half-life of functional unoxidized dextran-hemoglobin in the circulation was 1.9 days. In compensation for the loss of dextran-hemoglobin, vigorous erythropoiesis was observed at a rate of close to 5% hematocrit per day over the first 2 days following the exchange transfusion. As a result, the total hemoglobin concentration in blood was maintained at 5-6% during this period, and the animals went on to complete recovery in room air without the need for further transfusion with dextran-hemoglobin.
对犬进行换血治疗时，使用的是右旋糖酐溶液或右旋糖酐与人血红蛋白的共价复合物溶液。仅输注右旋糖酐的犬在血细胞比容降至6 - 10%时死亡。然而，输注右旋糖酐 - 血红蛋白复合物的犬在血细胞比容降至2%或更低时仍存活。在后一组动物中，右旋糖酐 - 血红蛋白复合物从循环中消失，平均半衰期为2.4天。校正血红蛋白部分氧化为高铁血红蛋白后，循环中功能性未氧化的右旋糖酐 - 血红蛋白的半衰期为1.9天。为了补偿右旋糖酐 - 血红蛋白的损失，在换血治疗后的头2天观察到旺盛的红细胞生成，速率接近每天血细胞比容增加5%。结果，在此期间血液中的总血红蛋白浓度维持在5 - 6%，并且这些动物在室内空气中继续完全康复，无需进一步输注右旋糖酐 - 血红蛋白。
