Wong S Y, Carrol E D, Ah-See S Y, Sewell H F
Department of Pathology, University Medical Buildings, Aberdeen, Scotland, United Kingdom.
Cancer. 1988 Nov 15;62(10):2171-5. doi: 10.1002/1097-0142(19881115)62:10<2171::aid-cncr2820621017>3.0.co;2-o.
A monoclonal antibody (anti-estrogen receptor [ER] from the ER-immunocytochemical assay [ICA] [Abbott Laboratories, Berkshire, England]), which binds to epitopes of the estrogen receptor in surgical biopsy specimens of breast carcinomas, was used in an improved alkaline phosphatase anti-alkaline phosphatase (APAAP) technique for in-situ receptor detection. This method was compared with a standard radioligand binding biochemical assay in assessing the ER status of 48 human breast carcinomas. The monoclonal antibody allowed detection of ER in the presence of estrogens or anti-estrogens and avoided problems of nonspecific intrinsic reactions, due to endogenous steroid hormones. The immunohistologic results (positive and negative) correlated well with the radioligand binding results (rs = 0.760; P less than 0.001) and allowed the additional assessment of cell morphology and tumor heterogeneity in the cryostat sections. The APAAP method provides diagnostic rapidity (approximately 4 hours) combined with sensitivity as compared with the radioligand assay (an approximately 2-day assay). The vivid red color associated with APAAP staining also was a distinct advantage compared with the brown reaction product of ER-ICA in interpreting stained sections. The results of this study indicate that the APAAP technique is an improved method for detection of ER and is applicable in routine hospital practice, thereby contributing to the immediate clinical management of patients with breast tumors.
一种单克隆抗体（来自雌激素受体免疫细胞化学检测[ICA]的抗雌激素受体[ER]，雅培实验室，英国伯克郡），它能与乳腺癌手术活检标本中雌激素受体的表位结合，被用于一种改良的碱性磷酸酶抗碱性磷酸酶（APAAP）技术进行原位受体检测。在评估48例人类乳腺癌的雌激素受体状态时，将该方法与标准的放射性配体结合生化检测进行了比较。该单克隆抗体能够在存在雌激素或抗雌激素的情况下检测雌激素受体，并且避免了由于内源性甾体激素导致的非特异性固有反应问题。免疫组织学结果（阳性和阴性）与放射性配体结合结果相关性良好（rs = 0.760；P < 0.001），并且能够在冰冻切片中额外评估细胞形态和肿瘤异质性。与放射性配体检测（大约需要2天的检测时间）相比，APAAP方法具有诊断快速性（大约4小时）和敏感性。与ER-ICA的棕色反应产物相比，APAAP染色产生的鲜艳红色在解释染色切片时也是一个明显的优势。本研究结果表明，APAAP技术是一种检测雌激素受体的改良方法，适用于医院常规实践，从而有助于对乳腺肿瘤患者进行即时临床管理。
