Person J M, Barrat F, Pilet C
Institut d'Immunologie Animale et Comparée, Ecole Nationale Vétérinaire d'Alfort, Maisons Alfort, France.
Comp Immunol Microbiol Infect Dis. 1988;11(2):125-37. doi: 10.1016/0147-9571(88)90028-8.
The identification and the numeration of the lymphocytes in cattle is realized essentially by revealing the membranary properties of these cells: receptors for some lectins (i.e. peanut agglutinin or phytohemagglutinin), receptors for sheep red blood cells (SRBC), surface-specific antigens concerning T-lymphocytes and surface immunoglobulins concerning B-lymphocytes. The technique of the production in the goat of a specific anti-bovine T-lymphocyte antiserum (GABTA) is described. The GABTA shows a specific cytotoxic activity on 55% of the peripheral blood lymphocytes and more than 95% of the thymocytes. It inhibits, at 90%, the formation of the rosetting with SRBC. This reagent allows identification and a common and quick numeration of the T-lymphocytes in cattle.
某些凝集素（即花生凝集素或植物血凝素）的受体、绵羊红细胞（SRBC）的受体、与T淋巴细胞相关的表面特异性抗原以及与B淋巴细胞相关的表面免疫球蛋白。本文描述了在山羊中制备特异性抗牛T淋巴细胞抗血清（GABTA）的技术。GABTA对55%的外周血淋巴细胞和95%以上的胸腺细胞具有特异性细胞毒活性。它对SRBC玫瑰花结形成的抑制率为90%。该试剂可用于牛T淋巴细胞的识别以及常规快速计数。
