Chandler J P, Yang T J
Int Arch Allergy Appl Immunol. 1981;65(1):62-8. doi: 10.1159/000232738.
A T-cell-specific rabbit anti-dog thymocyte antiserum was prepared and used in an indirect fluorescent antibody technique to identify T lymphocytes. The antiserum was rendered specific by absorptions with normal dog red blood cells, lyophilized serum, and bone marrow cells. By the indirect immunofluorescence assay, 73.6 +/- 1.2 (SEM)% of the peripheral blood lymphocytes were identified as T lymphocytes. By direct immunofluorescence assay of surface membrane immunoglobulin-positive lymphocytes, 18.2 +/- 0.8% of the peripheral blood lymphocytes were identified as B lymphocytes. The two assays used in conjunction can thus identify over 90% of the peripheral blood lymphocytes as either T or B lymphocytes, with the remaining 8.2 +/- 1.0% as null cells.
制备了一种针对T细胞的兔抗犬胸腺细胞抗血清，并将其用于间接荧光抗体技术以鉴定T淋巴细胞。通过用正常犬红细胞、冻干血清和骨髓细胞吸收，使抗血清具有特异性。通过间接免疫荧光测定，73.6±1.2（标准误）%的外周血淋巴细胞被鉴定为T淋巴细胞。通过对表面膜免疫球蛋白阳性淋巴细胞的直接免疫荧光测定，18.2±0.8%的外周血淋巴细胞被鉴定为B淋巴细胞。因此，联合使用这两种测定方法可将超过90%的外周血淋巴细胞鉴定为T或B淋巴细胞，其余8.2±1.0%为裸细胞。
