Schlegel R, Phelps W C, Zhang Y L, Barbosa M
National Cancer Institute, Bethesda, MD 20892.
EMBO J. 1988 Oct;7(10):3181-7. doi: 10.1002/j.1460-2075.1988.tb03185.x.
Keratinocytes electroporated with human papillomavirus (HPV) DNA (HPV-6, 11, 16 and 18) exhibited an increased cellular proliferation which was quantitated as microcolony and macrocolony formation. However, only macrocolonies induced by HPV-16 or HPV-18 DNA (the two viral types most commonly found in human cervical carcinomas) gave rise to proliferating, poorly-stratified colonies when grown in the presence of serum and calcium. Hydrocortisone increased the frequency of these differentiation-resistant colonies, and studies showed that they were immortalized, contained one copy of viral DNA per cell, expressed three discrete species of viral RNA and synthesized the viral E7 protein. HPV-induced cellular proliferation and altered differentiation are therefore separable events and may represent the activity of different viral genes.
用人乳头瘤病毒（HPV）DNA（HPV - 6、11、16和18型）进行电穿孔处理的角质形成细胞表现出细胞增殖增加，这可通过微集落和大集落形成进行定量。然而，只有由HPV - 16或HPV - 18 DNA（在人类宫颈癌中最常见的两种病毒类型）诱导形成的大集落，在血清和钙存在的情况下生长时，才会产生增殖性、分层不良的集落。氢化可的松增加了这些抗分化集落的频率，研究表明它们是永生化的，每个细胞含有一份病毒DNA拷贝，表达三种离散的病毒RNA种类，并合成病毒E7蛋白。因此，HPV诱导的细胞增殖和分化改变是可分离的事件，可能代表不同病毒基因的活性。
