Dürst M, Gissmann L, Ikenberg H, zur Hausen H
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3812-5. doi: 10.1073/pnas.80.12.3812.
DNA from one biopsy sample of invasive cancer of the cervix contained sequences hybridizing with human papillomavirus (HPV) type 11 DNA only under nonstringent conditions. This DNA was molecularly cloned in lambda phage. Under stringent conditions of hybridization it cross-hybridized to a minor extent (less than 0.1%) with HPV types 10, 14, and 15 and showed no homology with DNA of other human HPV types. We therefore propose to designate it tentatively as HPV 16. HPV 16 DNA was used as a probe to test additional cancer biopsy samples from cervical, vulval, and penile cancer, as well as benign genital warts (condylomata acuminata) and cervical dysplasias for the presence of homologous sequences. In 61.1% (11/18) of cervical cancer samples from German patients sequences were found hybridizing with HPV 16 DNA under conditions of high stringency. In contrast, only 34.8% (8/23) of cancer biopsy samples from Kenya and Brazil revealed this DNA. Vulval and penile cancer biopsy samples hybridized to 28.6% (2/7) or 25% (1/4), respectively. Only 2 out of 33 condylomata acuminata contained HPV 16 DNA. Both positive tumors harbored in addition HPV 6 or HPV 11 DNA. The data thus indicate that HPV 16 DNA prevails in malignant tumors, rendering an accidental contamination with papillomavirus DNA from adjacent papillomas rather unlikely. The rare presence in benign genital papillomas in addition to common genital papillomaviruses suggests a dependence of HPV 16 replication on helper virus.
来自一例宫颈浸润癌活检样本的DNA，仅在非严格条件下含有与人乳头瘤病毒（HPV）11型DNA杂交的序列。该DNA被分子克隆到λ噬菌体中。在严格杂交条件下，它与HPV 10、14和15型有少量交叉杂交（小于0.1%），与其他人类HPV型的DNA无同源性。因此，我们建议暂时将其命名为HPV 16。HPV 16 DNA用作探针，检测来自宫颈癌、外阴癌和阴茎癌的其他癌症活检样本，以及良性生殖器疣（尖锐湿疣）和宫颈发育异常中同源序列的存在情况。在德国患者的61.1%（11/18）宫颈癌样本中，在高严格条件下发现有与HPV 16 DNA杂交的序列。相比之下，来自肯尼亚和巴西的癌症活检样本中只有34.8%（8/23）显示有这种DNA。外阴癌和阴茎癌活检样本的杂交率分别为28.6%（2/7）或25%（1/4）。33例尖锐湿疣中只有2例含有HPV 16 DNA。两个阳性肿瘤还同时含有HPV 6或HPV 11 DNA。因此，数据表明HPV 16 DNA在恶性肿瘤中占优势，不太可能是来自相邻乳头瘤的乳头瘤病毒DNA偶然污染所致。除常见的生殖器乳头瘤病毒外，HPV 16在良性生殖器乳头瘤中罕见存在，提示HPV 16的复制依赖辅助病毒。
